We narrowed to 4,410 results for: Erf

Showing: 3471 - 3480 of 4410 results

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pBbS8c-ddcpf1-Δ

Plasmid

#153039

Purpose

For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.

Depositor

Nigel Scrutton

Article

Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

Insert

Ascpf1

Expression

Bacterial

Mutation

E993A

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-FOLD6

Plasmid

#186547

Purpose

FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

FOLD6

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

Promoter

araB

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sgempty

Plasmid

#159523

Purpose

Expression of dCas9 driven by lux cassette from Vibrio fischeri

Depositor

Radhakrishnan Mahadevan

Article

Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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4xHRE_minCMV-sfGFP-CMV_dsRed Exp

Plasmid

#117401

Purpose

sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Expression

Mammalian

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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lenti GFAABC1D-dCas9-KRAB-MeCP2

Plasmid

#194701

Purpose

Expresses dCas9-KRAB-MeCP2 in Astrocytes

Depositor

Timothy Jarome

Article

Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

Insert

GFAABC1D promoter

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

None

Promoter

GFAABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-552

Plasmid

#74614

Purpose

Expresses a truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-552 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9+sgRNA expression vector precursor

Plasmid

#171798

Purpose

PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

Depositor

Stephen Elledge

Article

Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

pLtetO

Available Since

Aug. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9-NED

Plasmid

#109358

Purpose

Mammalian vector for expressing effector domains fused to dCas9.

Depositor

Antal Kiss

Article

Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

Type

Empty backbone

Use

CRISPR

Tags

dCas9, SV40 NLS, 3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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BB1_23_dCAS9(3xFLAG_SV40NLS)

Plasmid

#161478

Purpose

BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Insert

dCAS9

Use

CRISPR and Synthetic Biology

Tags

FLAG Tag, SV40 NLS

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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