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Showing: 3471 - 3480 of 158806 results

  1. pRBC-sfGFP-150TAG

    Plasmid
    #174076
    Purpose
    Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,
    Depositor
    Ryan Mehl
    Article
    Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15
    Insert
    Superfolder GFP
    Use
    Tags
    His-6
    Expression
    Bacterial
    Mutation
    N150TAG
    Promoter
    T7
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    174076-plasmid-map-sequence-id-345697

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  3. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  4. His-AgdNK

    Plasmid
    #203226
    Purpose
    For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.
    Depositor
    Vern Schramm
    Article
    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi
    Insert
    deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
    Use
    Tags
    N-terminal TEV cleavable 6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203226-plasmid-map-sequence-id-403332

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  10. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6 in tandem
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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Showing: 3471 - 3480 of 158806 results