We narrowed to 13,360 results for: Mpl;

1. myc-Atg14 (1-181)

   Plasmid

   #86752

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   atg14 (ATG14 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   aa 1-181

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJZC620

   Plasmid

   #62282

   Purpose

   Expresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   PCP-VP64
   dCas9
   

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Nuclease activity has been inactivated by mutatio…

   Promoter

   pAdh and pTdh3

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-Nsp5-HF

   Plasmid

   #157687

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp5-HF (ORF1ab SARS-CoV-2)
   

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3 βarr1 S412D Flag

   Plasmid

   #42196

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   β-arrestin 1 S412D (Arrb1 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S412D

   Promoter

   CMV

   Available Since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

5. OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

   Plasmid

   #128010

   Purpose

   traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
   

   Use

   Drosophila osc reporter construct

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fyn-SH3_5WT_S25C

   Plasmid

   #118824

   Purpose

   Bacterial expression of the SH3 domain of human Fyn

   Depositor

   Nicolas Fawzi

   Article

   Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

   Insert

   FYN (FYN Human)
   

   Tags

   6-His-TEV tag

   Expression

   Bacterial

   Mutation

   S25C; See depositor comments below.

   Available Since

   April 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.3−3xFLAG−AGO2(S387D)

   Plasmid

   #220463

   Purpose

   Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

   Depositor

   David Bartel

   Article

   Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

   Insert

   Human AGO2 (AGO2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Serine 387 to aspartate

   Promoter

   CMV

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. myc-Atg14 (1-200)

   Plasmid

   #86750

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

   Insert

   Atg14 (ATG14 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   aa 1-200

   Promoter

   CMV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNS19-LV-mutRFP-2A-GFP

   Plasmid

   #113716

   Purpose

   Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

   Depositor

   Gerald Schwank

   Article

   Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

   Insert

   mutRFP-2A-GFP-IRES-Puro
   

   Use

   Lentiviral; Reporter system

   Expression

   Mammalian

   Mutation

   Y64L (mutRFP)

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTag-RFP-C-h-Rab4a-c-Myc

    Plasmid

    #79800

    Purpose

    Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab4a (RAB4A Human)
    

    Tags

    TagRFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. hnRNPA2_266-341

    Plasmid

    #118821

    Purpose

    Bacterial expression of hnRNPA2 truncation

    Depositor

    Nicolas Fawzi

    Article

    Amaya et al J Biol Chem. 2018 Dec 21;293(51):19522-1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 266-341

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4.TO-ORF62-2xCSTREP

    Plasmid

    #136222

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF62

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF62
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA4.TO-ORF6-2xCSTREP

    Plasmid

    #136170

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF6

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro-HA-PIK3CA(H1047R;I848V)

    Plasmid

    #33253

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and isoleucine…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-FRT-TO-FH-Nsp13

    Plasmid

    #157713

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13 (ORF1ab SARS-CoV-2)
    

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDESTmycEIF2C2

    Plasmid

    #19872

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3-V5-PTPN14-PPxY

    Plasmid

    #61006

    Purpose

    Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 PPxY mutation (PTPN14 Human)
    

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    PPxY mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA4.TO-ORF74-2xNSTREP

    Plasmid

    #136233

    Purpose

    Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF74
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFL His-mEos3.2-I3

    Plasmid

    #113516

    Purpose

    Expression of His-Eos-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Tags

    6*His-mEos3.2

    Expression

    Insect

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-D4 miR30-DNMT3a ts1

    Plasmid

    #115865

    Purpose

    DNMT3a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    DNMT3a shRNA (DNMT3A Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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