4,183 results

1. pBabe-Puro-Myr-Flag-AKT1

   Plasmid

   #15294

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   Myr-Flag

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. dxCas(3.7)-VPR

   Plasmid

   #108383

   Purpose

   Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.7)-VPR
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CA-rtTA Adv

   Plasmid

   #20910

   Purpose

   piggyBac vector for expression of reverse tetracycline transactivator from a CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   rtTA-ON-Adv
   

   Use

   piggybac

   Expression

   Mammalian

   Available Since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGADCg

   Plasmid

   #20161

   Depositor

   Peter Uetz

   Article

   Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

   Type

   Empty backbone

   Tags

   Gal4 AD and SV40 NLS

   Expression

   Yeast

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACUH-GFP11x7-mCherry-α-tubulin

   Plasmid

   #70218

   Purpose

   Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP11x7-mCherry-α-tubulin
   

   Tags

   GFP11x7 and mCherry

   Expression

   Insect

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJ241-FLEX

   Plasmid

   #18925

   Purpose

   FLEX switch empty backbone

   Depositor

   Scott Sternson

   Article

   Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

   Insert

   FLEX switch
   

   Use

   Cre/Lox; Subcloning

   Mutation

   An internal Spe1 site is used for subcloning a ge…

   Available Since

   Jan. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-dCas9 VP64-T2A-GFP

   Plasmid

   #53192

   Purpose

   Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 VP64 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIS2

   Plasmid

   #12177

   Depositor

   David Bartel

   Article

   Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK7WGF2::hCas9

   Plasmid

   #46965

   Purpose

   Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   hCas9
   

   Use

   CRISPR; Plant expression

   Tags

   eGFP

   Promoter

   35S

   Available Since

   Aug. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag pLJM1 RagB wt

    Plasmid

    #19313

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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