12,939 results

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pORANGE Cacnb4-GFP KI

Plasmid

#139663

Purpose

Endogenous tagging of CaVβ4: C-terminal (amino acid position: H417)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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mEmerald-TMEM216-N-18

Plasmid

#54280

Purpose

Localization: Cilium, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

TMEM216 (Tmem216 Mouse)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Px458-Cas9n-Trp63-Exon4-2sgRNA

Plasmid

#88849

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63 (Trp63 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
pLL3.7-Casp2-shRNA

Plasmid

#157926

Purpose

Knockdown of caspase-2

Depositor

Baoji Xu

Article

Xu et al Nat Commun. 2019 Aug 9;10(1):3622. doi:

Insert

Casp2 shRNA (Casp2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Promoter

mouse U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-F-Gcn5-NLSm

Plasmid

#63708

Purpose

retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

mutated K425, K427 and K438 to R (arginine)

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-Trib1

Plasmid

#131156

Purpose

Mammalian expression of flag-tagged mouse TRIB1

Depositor

Karen Keeshan, Warren Pear

Article

Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

Insert

Trib1 (Trib1 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_ACTB CRISPIE

Plasmid

#172853

Purpose

Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Inserts

Actb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRNATU6.1 HDAC3 shRNA2

Plasmid

#112271

Purpose

HDAC3 KD

Depositor

Simone Di Giovanni

Insert

shRNA against mouse HDAC3 (Hdac3 Mouse)

Tags

GFP

Expression

Mammalian

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-Rheb SSVM-IP

Plasmid

#13832

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb SSVM (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C181 changed to serine

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
Slax mMyf5 (CT#1008)

Plasmid

#13913

Depositor

Cliff Tabin

Article

Brent et al Development. 2005 Feb . 132(3):515-28.

Insert

Myf5 (Myf5 Mouse)

Expression

Bacterial

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
gfp-Arkadia/ RNF111 deltaRING

Plasmid

#112229

Purpose

Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cells

Depositor

Vasso Episkopou

Article

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta RING domain deletion (Deletion of the last …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only
pmTUBB3

Plasmid

#31236

Depositor

Cliff Ragsdale

Article

Grove et al Development. 1998 Jun . 125(12):2315-25.

Insert

TUBB3 (Tubb3 Mouse)

Use

Cdna for in situ hybridization

Mutation

3'UTR sequence

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
CD8a-f-CTS-EGFP

Plasmid

#86063

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

contains CD8a luminal and transmembrane domains a…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4

Plasmid

#112839

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Grb2 (Grb2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

SH3 (N-term) domain of Grb2

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pROSA26-G19

Plasmid

#21711

Depositor

Philippe Soriano

Article

Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

Insert

ROSA26 G19 (Gt(ROSA)26Sor Mouse)

Expression

Bacterial

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Human, Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Promoter

LTR

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-Profilin-10

Plasmid

#57414

Purpose

Localization: Cytoskeleton, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Profilin

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
bU6-sgNeo1-mU6-sgNT-hU6-sgSnrk

Plasmid

#177236

Purpose

Expresses neomycin (bU6), non-targeting (mU6) and Snrk (hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNT1/sgSnrk

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

Plasmid

#177234

Purpose

Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgNuak1/sgNuak2

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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bU6-sgNeo1-mU6-sgAmpk1-hU6-sgAmpk2

Plasmid

#177233

Purpose

Expresses Neomycin (bU6), Ampk1 (mU6) and Ampk2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Insert

sgNeo1/sgPrkaa1/sgPrkaa2

Use

Lentiviral

Promoter

bU6/mU6/hU6

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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