We narrowed to 1,322 results for: pET28a

1. HTATIP (2OU2)

   Plasmid

   #33338

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HTATIP (KAT5 Human)
   

   Use

   Tags

   Hexahistidine tag with Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pET_mNG2(1-10)_32aalinker_mNG2(11)

   Plasmid

   #82611

   Purpose

   Bacterial expression vector for split mNeonGreen2 screening

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   mNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   split before mutations

   Promoter

   T7

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-Ran(Q69L)

   Plasmid

   #42048

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Ran (RAN Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Q69L

   Promoter

   Available Since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Use

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28_hTLN2-FL

   Plasmid

   #164835

   Purpose

   Expression of FL human talin2

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

   Insert

   TLN2 (TLN2 Human)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_mouse-Caspase-11_enzymatic-domain

   Plasmid

   #183382

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-11 (AA100-373) (Casp4 Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIA1364

   Plasmid

   #172401

   Purpose

   Expression of SARS-COV-2 Nsp9 (extra GS at the N-terminal) in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp9 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. tIVR

   Plasmid

   #122663

   Purpose

   Express tIVR protein in E. coli

   Depositor

   Robert Cohen

   Article

   Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Insert

   tIVR
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pET_scFv_QLuc

   Plasmid

   #183261

   Purpose

   Express scFv_QLuc in bacteria cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   scFv_QLuc
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK438

    Plasmid

    #71590

    Purpose

    Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)

    Depositor

    T. J. Kappock

    Article

    Murphy et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-SUMO-KaiB

    Plasmid

    #68050

    Purpose

    Strain: AM5116, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiB
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-SUMO-KaiA

    Plasmid

    #68049

    Purpose

    Strain: AM5115, Used for protein purification

    Depositor

    Susan Golden

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 Oct 30;10

    Insert

    KaiA
    

    Use

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-28a-CylA

    Plasmid

    #48198

    Purpose

    Expresses CylA (fatty acyl-AMP ligase) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28-p53 (Dog 1-77)

    Plasmid

    #62060

    Purpose

    Expression of Dog p53 (residues 1-77) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-77

    Promoter

    T7

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. POC1479

    Plasmid

    #213805

    Purpose

    BsaI-associated non-switchable methylase

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    Non-switchable methylase M1.Eco31I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. POC1480

    Plasmid

    #213806

    Purpose

    BsaI-associated non-switchable methylase

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    Non-switchable methylase M1.Eco31I
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. POC1483

    Plasmid

    #213809

    Purpose

    BpiI-associated non-switchable methylase

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Insert

    BpiI-associated non-switchable methylase M1.HpyAII
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_canine-Caspase-1/4_enzymatic-domain

    Plasmid

    #183383

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_hTLN2-head

    Plasmid

    #164836

    Purpose

    Expression of human talin2 head

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28-p53 (Cow 1-82)

    Plasmid

    #62062

    Purpose

    Expression of Cow p53 (residues 1-82) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-82

    Promoter

    T7

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pET28-p53 (Rabbit 1-87)

    Plasmid

    #62058

    Purpose

    Expression of Rabbit p53 (residues 1-87) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

    Insert

    p53
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-87

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pET-28a-CylI

    Plasmid

    #48200

    Purpose

    Expresses CylI (type III PKS) in E. coli

    Depositor

    Emily Balskus

    Article

    Nakamura et al J Am Chem Soc. 2012 Nov 14;134(45):18518

    Insert

    CylI
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pA9HD

    Plasmid

    #8588

    Depositor

    Cynthia Wolberger

    Article

    LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

    Insert

    HoxA9 190-271with hexapeptide (HOXA9 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

25. SpyTag003-sfGFP

    Plasmid

    #133454

    Purpose

    Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-sfGFP
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SpyTag003-mKate2

    Plasmid

    #133452

    Purpose

    Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mKate2
    

    Use

    Tags

    GSGSGS linker and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBECAb-apr

    Plasmid

    #122001

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET_sfCherry(1-10)_32aalinker_sfCherry(11)

    Plasmid

    #83030

    Purpose

    Bacterial expression vector for split sfCherry screening

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry(1-10)_32aalinker_sfCherry(11)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDR119

    Plasmid

    #13332

    Depositor

    Martin Gellert

    Article

    Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

    Insert

    DNA ligase IV (LIG4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

30. Human USP30 (64-502, construct 2, MG-26-18)

    Plasmid

    #110744

    Purpose

    Bacterial expression of human USP30 (construct 2)

    Depositor

    David Komander

    Article

    Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

    Insert

    USP30 (USP30 Human)
    

    Use

    Tags

    N-His6-3C-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge