We narrowed to 1,198 results for: e.coli
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Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVRa14_3200
Plasmid#49666DepositorInsertECF14_3200
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1158
Plasmid#53007PurposepLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
DsrA sRNA
ompC
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains -56 to +141 relative to start codon and …PromoterpCon, pLlacO-1, and pLtetO-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHYP-NPC-10H
Plasmid#162789PurposeSARS-CoV-2 Nucleocapsid Protein expression in E.coliDepositorInsertNucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
Tags10xHisExpressionBacterialPromoterT7lacAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
Plasmid#24549DepositorInsertpADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-Aurein1.2_(+36GFP)-LPETG-His
Plasmid#71755PurposeHis-TEV-Aurein1.2+36GFP-LPETG-His expression in E.ColiDepositorInsertAurein1.2_(pos36_GFP)-LPETG-His
Tags6XHis, Sortase (LPETG), and TEV ProteaseExpressionBacterialAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa41_491
Plasmid#49702DepositorInsertECF41_491
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the TM287/288 nanobody Nb_TM#2
Plasmid#132698Purposeexpresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISADepositorInsertnanobody Nb_TM#2
ExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDI
Plasmid#60555Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneTagsHis6-hPDIExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTYF-2xSYN-tTA
Plasmid#19982DepositorInsert2xSYN-tTA (SYN1 Human, Budding Yeast, E.coli)
ExpressionMammalianAvailable SinceAug. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneTagsHis6-hPDIb'a' tagExpressionBacterialAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
ExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRa16_3622
Plasmid#49670DepositorInsertECF16_3622
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc03_1198
Plasmid#49730DepositorInsertAS03_1198
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXPHM3-PCFT_NB
Plasmid#165415PurposeExpression in E.coli of N terminally MBP tagged anti-GgPCFT nano bodyDepositorInsertGgPCFT_NB (SLC46A1 Chicken, Llama)
TagsHis10-MBP-3C protease siteExpressionBacterialPromoterarabinoseAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin W403A
Plasmid#45974DepositorInsertParkin W403A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_D27NDHFR_WTTS
Plasmid#91244Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationD27NPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31YG121VDHFR_WTTS
Plasmid#91229Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
Tags6xHisExpressionBacterialMutationF31YG121VPromoterT7Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only