We narrowed to 55,039 results for: tra

1. UbC-blasticidine-P2A-TETon-3G transactivator

   Plasmid

   #200191

   Purpose

   A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   blasticidine - P2A - TETon-3G transactivator
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Promoter

   UbC

   Available Since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv1.4 K+ channel (extracellular) scFv [L71/5]

   Plasmid

   #190517

   Purpose

   Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL4.23-CD69 CaRE3 (minus strand)

   Plasmid

   #91845

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0622)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE3 (minus strand) (CD69 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Missing 1 base from 13 base polyT tract beginning…

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Anti-LRP4 (extracellular) [N207/27R]

   Plasmid

   #177513

   Purpose

   Mammalian Expression Plasmid of anti-LRP4 (extracellular) (Mouse). Derived from hybridoma N207/27.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-LRP4 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Lrp4 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGal-Trans-Myc-eGFP-KDEL

   Plasmid

   #177729

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Tags

   KDEL, Myc, and eGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Gene Trap - 24XMS2 Lentiviral Vector

   Plasmid

   #174198

   Purpose

   Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

   Depositor

   Daniel Larson

   Article

   Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

   Insert

   iRFP
   

   Use

   Lentiviral

   Tags

   24X MS2 hairpins, Blasticidin, and T2A

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAD-LyseR (in lacZ deficient strain)

   Plasmid

   #99245

   Purpose

   For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Expression

   Bacterial

   Promoter

   beta-lactamase promoter

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. OCT4-GFP-2A-PURO transgenic reporter

   Plasmid

   #52379

   Purpose

   BAC recombineering generated construct, used as a reporter for OCT4 expression

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Inserts

   GFP-2A-puro
   OCT4
   

   Expression

   Mammalian

   Promoter

   OCT4

   Available Since

   Oct. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Plk1 FRET sensor c-jun substrate

    Plasmid

    #45203

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Plk1 FRET sensor with c-jun substrate
    

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAd-Track Flag HA PGC-1 alpha

    Plasmid

    #14426

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL16

    Plasmid

    #158742

    Purpose

    Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Neuraminidase A/NL/354/16 H3N2
    

    Tags

    TwinStrep_sfGFP_tetrabrachion

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTol2-nbt-Electra2-P2A-mScarlet

    Plasmid

    #196114

    Purpose

    zebrafish expression of nbt-Electra2-P2A-mScarlet

    Depositor

    Reinhard Köster

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    nbt-Electra2-P2A-mScarlet
    

    Use

    Zebrafish expression

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

    Plasmid

    #211817

    Purpose

    AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    N-terminus of SpCas9-ABE8e and tracrRNA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter and U6 promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Pan-Neurofascin (extracellular) scFv [A12/18]

    Plasmid

    #190485

    Purpose

    Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23-IL2RA CaRE4 (plus strand)

    Plasmid

    #91850

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0345)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET co-transformation cloning vector (13S-A)

    Plasmid

    #48323

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.23-IL2RA CaRE4 (minus strand)

    Plasmid

    #91849

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0626)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAd-Track Flag-SIRT1 H355A

    Plasmid

    #8439

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Histidine 355 mutated to Alanine

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge