We narrowed to 7,668 results for: CCH

1. YIp128-CUP1-His6-CUbo(K63R)-GFP

   Plasmid

   #212794

   Purpose

   Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   His6-CUbo(K63R)-GFP
   

   Use

   Integrative vector

   Expression

   Yeast

   Mutation

   CUb(K63R)

   Promoter

   CUP1

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. YIp211-ADH-NUbo-E3(48) aa24-150-VSV

   Plasmid

   #212747

   Purpose

   Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(48) aa24-150
   

   Use

   Integrative vector

   Tags

   NUbo and VSV

   Expression

   Yeast

   Mutation

   Cue1 ΔU7BD

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

   Plasmid

   #212738

   Purpose

   Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   E3(1)
   

   Use

   Integrative vector

   Tags

   CUbo-NLS-VSV and myc

   Expression

   Yeast

   Mutation

   HOIP(C885A)

   Promoter

   ADH1

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry

   Plasmid

   #202090

   Purpose

   Neuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutations

   Depositor

   Elizabeth Heller

   Article

   Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

   Insert

   dCas9-Set2(R195G, C201A)
   

   Tags

   3x FLAG and mCherry

   Expression

   Mammalian

   Mutation

   R195G and C201A abolish catalytic activity

   Promoter

   human Synapsin

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

   Plasmid

   #212805

   Purpose

   Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
   

   Expression

   Mammalian

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN HA-ATG13(Δ1-198)

   Plasmid

   #186222

   Purpose

   For expression of ATG13(Δ1-198) tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1 - 198

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-GST-TEV-mCherry-TBK1

   Plasmid

   #198033

   Purpose

   Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Insect

   Mutation

   E696K

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GA46

   Plasmid

   #196614

   Purpose

   Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

   Depositor

   Matthias Heinemann

   Article

   Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

   Insert

   ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
   

   Tags

   AID(71-114) and mCherry

   Expression

   Yeast

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

    Plasmid

    #185346

    Purpose

    Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Cue1p/Ube2G2 fusion (internal MYC tag)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

    Plasmid

    #185347

    Purpose

    Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Cue1p/Ube2G2 fusion (internal MYC tag)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid

    #184774

    Purpose

    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Budding Yeast, Mouse)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGS_591

    Plasmid

    #160653

    Purpose

    Expresses OLE1 under the control of the yeast Sup35 promoter

    Depositor

    Susan Lindquist, Li-Huei Tsai

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    YGL055W (OLE1 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    Sup35 promoter

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Linked VLP NES

    Plasmid

    #182432

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Linked VLP NES (M)

    Plasmid

    #182434

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(M)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Linked VLP NES (G)

    Plasmid

    #182476

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-mCherry-SynaptoZip

    Plasmid

    #177318

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBICI_high ori

    Plasmid

    #179273

    Purpose

    Expresses CuCBP and BaSP in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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