We narrowed to 16,357 results for: Jun

1. CMV10-3xFlag Skp2 delta-F

   Plasmid

   #81116

   Purpose

   mammalian expression of F-box deleted Skp2

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   deleted F box

   Promoter

   CMV

   Available since

   Sept. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

2. GFP-Tollip M240A,F241A

   Plasmid

   #178051

   Purpose

   GFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Methionine 240 to Alanine and changed Phe…

   Promoter

   CMV

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBB COMMD1 Flag

   Plasmid

   #74893

   Purpose

   Express COMMD1 in mammalian cells

   Depositor

   Colin Duckett, Ezra Burstein

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD1 (COMMD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-myc-Miro1 Δ593-618

   Plasmid

   #47895

   Purpose

   Expresses myc tagged Miro1 lacking transmembrane domain

   Depositor

   Pontus Aspenström

   Article

   Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

   Insert

   Miro1 Δ593-618 (RHOT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Δ593-618, lacks transmembrane domain

   Promoter

   CMV

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

5. LSB-hsa-miR-181a-5p

   Plasmid

   #103276

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-181a-5p target
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR366 RNF2

   Plasmid

   #47509

   Purpose

   human gRNA expression vector targeting RNF2

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-RNF2 (RNF2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330 _CRISPIE donor B10

   Plasmid

   #172851

   Purpose

   DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

   Plasmid

   #99229

   Purpose

   Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos2
   

   Use

   Tags

   Lifeact

   Expression

   Mammalian

   Mutation

   A69T for primed conversion applications

   Promoter

   CMV

   Available since

   Oct. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-nHyPer:cAPX

   Plasmid

   #84736

   Purpose

   Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   APX1 (APX1 Mustard Weed)
   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS-SV40

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dLight1.5

    Plasmid

    #111058

    Purpose

    Expresses dLight1.5 in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.5
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1 kb Axin2 basic

    Plasmid

    #25701

    Depositor

    Eric Fearon

    Article

    Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

    Insert

    Axin 2 promoter (AXIN2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pD21-hgAPOAI

    Plasmid

    #124962

    Purpose

    Express ApoAI in the liver

    Depositor

    Kazuhiro Oka

    Article

    Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

    Insert

    human APOAI gene (LPA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    human APOAI

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX Tec-SH2

    Plasmid

    #46522

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tec (TEC Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 236-351

    Promoter

    Tac

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. LADL Anchor

    Plasmid

    #127664

    Purpose

    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    dCas9-CIBN-2A-Puro
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 (D10A,H840A) mutant

    Promoter

    EF1a

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

    Plasmid

    #74261

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.Y591X truncation

    Promoter

    CMV

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid

    #172670

    Purpose

    For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    SDHB pegRNA and SDHB_nick-sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcdna DSG-2 Tailless

    Plasmid

    #89466

    Purpose

    DSG-2 tailless control

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. HA-VHL Y98H-pRc/CMV

    Plasmid

    #22044

    Depositor

    William Kaelin

    Article

    Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    point mutant, converting aa 98 from Tyrosine to H…

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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