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pAAVf-EnhCB-lacZnls

Plasmid

#35642

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3012

Plasmid

#91045

Purpose

Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

PvUbi1

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB6577

Plasmid

#106129

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pZEG

Plasmid

#129112

Purpose

Z. rouxii episomal vector containing G418 resistance

Depositor

Paolo Giudici

Article

Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Expression

Bacterial

Promoter

J23150

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTRANS_110

Plasmid

#91202

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaHFm

Plasmid

#85103

Purpose

Heme-FMN domain of P450 BM3 variant (A74G)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Heme-FMN domain of P450 BM3

Use

Cloning vector

Mutation

A74G

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB5219

Plasmid

#106135

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRoTnsABCDQ

Plasmid

#178772

Purpose

Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-3HA

Plasmid

#17373

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3HA (WNT3 Mouse, Human)

Tags

HA

Expression

Bacterial

Mutation

This gene is a fusion of the murine and human wnt…

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

D10A mutation; NLS fusion

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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tdEos-Paxillin-KM-C-10

Plasmid

#57654

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin KM

Tags

tdEos

Expression

Mammalian

Mutation

Mutation to remove Kozak sequence

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pc3XB-ZF70

Plasmid

#13193

Depositor

Daniel Voytas

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF70

Expression

Mammalian

Available Since

Oct. 31, 2006

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6G-J23106-geneIII

Plasmid

#80906

Purpose

Phagemid (encodes cI5G6G and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T-J23106-geneIII

Plasmid

#80907

Purpose

Phagemid (encodes cI5G6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T-J23106-geneIII

Plasmid

#80908

Purpose

Phagemid (encodes cI4A5T6T and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-GFP-2xmiR-34 (pAW-AS-25)

Plasmid

#40833

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Two target sites perfectly complementary to miR-34

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pI4-MTU-DO-HIS

Plasmid

#160026

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HPH

Plasmid

#160019

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-LEU

Plasmid

#160017

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A3010

Plasmid

#91044

Purpose

Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Promoter

ZmUbi

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pHFX-cas3UD

Plasmid

#174385

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

Cas3UD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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OptoOFF-TtCBD-mFGFR1_624

Plasmid

#105826

Purpose

Expresses OptoOFF-TtCBD-mFGFR1 (light-inactivated receptor tyrosine kinase)

Depositor

Harald Janovjak

Article

Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

Insert

OptoOFF-TtCBD-mFGFR1

Expression

Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMPRMA38

Plasmid

#172937

Purpose

pQE30 encoding Wzz mutant i92

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i92 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA48

Plasmid

#172941

Purpose

pQE30 encoding Wzz mutant i131

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i131 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA50

Plasmid

#172936

Purpose

pQE30 encoding Wzz mutant i219

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i219 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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VV247: sdChR(C138S)-TS-GCaMP6f-ER in fck

Plasmid

#58522

Purpose

Expresses sdChR(C138S) fused to GCaMP6f in mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

sdChR(C138S)-TS-GCaMP6f-ER

Use

Lentiviral

Tags

GCaMP6f

Expression

Mammalian

Mutation

changed Cysteine 138 to Serine

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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