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pHT-trxA.ads

Plasmid

#47383

Purpose

Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteria

Depositor

Heng-Phon Too

Article

Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

Insert

ads

Tags

6xHis and trxA

Expression

Bacterial

Promoter

Pgrac

Available Since

Oct. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pTTP1A

Plasmid

#91721

Purpose

integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

Depositor

Graham Hatfull

Article

Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

Type

Empty backbone

Use

Integration-proficient plasmid vectors for mycoba…

Available Since

May 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA3-TSC1

Plasmid

#19911

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

HA

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJB2a

Plasmid

#19550

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2a

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pX330_DDX4-cterm

Plasmid

#222520

Purpose

Cas9/sgRNA plasmid for targeting DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, DDX4 sgRNA (DDX4 Synthetic, Human)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-mEOS3.2-KanMX6

Plasmid

#87030

Purpose

Tag S. pombe genes in C terminal with mEOS3.2

Depositor

Julien Berro, Thomas Pollard

Article

Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

mEOS3.2

Expression

Bacterial and Yeast

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-C1-PLCdelta-PH

Plasmid

#21262

Depositor

Tobias Meyer

Article

Botelho et al J Cell Biol. 2000 Dec 25. 151(7):1353-68

Insert

PLCdelta (PLCD1 Human)

Tags

CFP

Expression

Mammalian

Mutation

PH domain of PLCdelta (human). Also known as PLCd…

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Article

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMC2SG5

Plasmid

#31283

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry2 (Cry2 Mouse)

Tags

Flag, His, and Myc

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
TRPML3-YFP

Plasmid

#18835

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

YFP

Expression

Mammalian

Mutation

Q165R, F210Y

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pJK_kanR_proJK1_PAD

Plasmid

#60630

Purpose

Constitutively expresses PAD (phenolic acid decarboxylase)

Depositor

Timothy Whitehead

Article

Tomek et al Biotechnol Bioeng. 2015 Mar;112(3):627-3

Insert

Phenolic acid decarboxylase padC (padC )

Expression

Bacterial

Mutation

K146Y

Promoter

proJK1

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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ZipGFP1-10_TEV

Plasmid

#81242

Purpose

Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

zipped GFP11 with TEV cleavage sequence T2A mCherry

Expression

Mammalian

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416GPD-Cerulean-YPT7

Plasmid

#18847

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

YPT7 (YPT7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMaCTag-23

Plasmid

#120002

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with Halo

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA2

Plasmid

#101042

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Article

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA2

Tags

GST

Expression

Bacterial

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ274 (pLEU MET25pro MCP-2x-yeGFP)

Plasmid

#45929

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

MCP-2x-yeGFP

Tags

2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-hph-Para

Plasmid

#218363

Purpose

Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL1P1_AtU626Ter26

Plasmid

#191769

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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