165,307 results

1. pTHSSe_45

   Plasmid

   #109243

   Purpose

   pSC101 var3 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Expression

   Bacterial

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

   Plasmid

   #114070

   Purpose

   T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
   

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-MND-hCard11 WT-T2A-GFP

   Plasmid

   #163787

   Purpose

   Expression of Wild-type CARD11

   Depositor

   Richard James

   Article

   Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

   Insert

   CARD11 (CARD11 Human)
   

   Use

   Lentiviral

   Promoter

   MND

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pClneoMyc-LATS2

   Plasmid

   #66852

   Purpose

   Express Myc-tagged LATS2 in mammalian cells

   Depositor

   Yutaka Hata

   Article

   Bao et al J Biochem. 2011 Aug;150(2):199-208. doi:

   Insert

   LATS2 (LATS2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINB-OTUB1*

   Plasmid

   #65441

   Purpose

   Expresses activated OTUB1 in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   UbcH5b (UBE2D2 Human)
   otubain-1 (OTUB1 )
   

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Active site cysteine mutated to alanine

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

6. Lenti-sgStk11#2/Cre

   Plasmid

   #173653

   Purpose

   Expresses a Stk11-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Stk11 (Stk11 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EYFP Fibronectin

   Plasmid

   #56600

   Purpose

   Localization: Extracellular Matrix, Excitation: 513, Emission: 527

   Depositor

   Michael Davidson

   Insert

   Fibronectin
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Fibronectin begins amino acids at amino acid 900 …

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. Flag-neo-PP1-Gamma

   Plasmid

   #171270

   Purpose

   Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1

   Depositor

   Steven Balk

   Insert

   3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-ATG5-K130R

   Plasmid

   #13096

   Depositor

   Roberta Gottlieb

   Article

   Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

   Insert

   ATG5-K130R (Atg5 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K130R

   Available Since

   Nov. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Bak

    Plasmid

    #32564

    Depositor

    Richard Youle

    Article

    Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

    Insert

    human Bak (BAK1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. LD-RA

    Plasmid

    #209863

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF0861

    Plasmid

    #141740

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H4 (NR1H4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-CMV-KIF2C

    Plasmid

    #205994

    Purpose

    Over-expression of GFP-KIF2c

    Depositor

    Floris Foijer

    Article

    Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

    Insert

    KIF2c (KIF2C Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-AAV-syn-iGABASnFR2n-WPRE

    Plasmid

    #218875

    Purpose

    AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F104H R168P

    Promoter

    Synapsin

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJKR-H-tetR

    Plasmid

    #62561

    Purpose

    Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-mTurquoise2-CNA35

    Plasmid

    #61602

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mTurqouise and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.DEST.YFP

    Plasmid

    #32683

    Purpose

    Lentiviral cDNA/shRNA gateway destination vectors

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF146 pSOD1WTAcGFP1

    Plasmid

    #26407

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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21. p2R3a-Tq2CFP-OcsT

    Plasmid

    #71268

    Purpose

    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pcDNA3/Flag-METTL14

    Plasmid

    #53740

    Purpose

    For expression of METTL14 (methyltransferase-like 14) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Methyltransferase-like 14 (METTL14 Human)
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. eDrFGFR1

    Plasmid

    #183994

    Purpose

    Optically controlled eDrFGFR1 expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrFGFR1 (FGFR1 Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRE118

    Plasmid

    #43830

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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27. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pTHSSe_47

    Plasmid

    #109245

    Purpose

    pSC101 var5 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBN41

    Plasmid

    #86716

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-2::GFP
    

    Expression

    Worm

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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