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169,885 results

Showing: 10561 - 10590 of 169885 results

  1. ADRB3-DuET

    Plasmid
    #213182
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    ADRB3 (ADRB3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213182-plasmid-map-sequence-id-430749

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  3. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  4. pDule-para-aminoPhe

    Plasmid
    #85502
    Purpose
    Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.
    Insert
    p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32T, E107T, D158P, I159L, L162A
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85502-plasmid-map-sequence-id-169860

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  6. pcDNA3.1-ccdB-V5

    Plasmid
    #214985
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    V5
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214985-plasmid-map-sequence-id-440020

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  27. p5E-mpeg1.1

    Plasmid
    #75023
    Purpose
    Can be used to drive expression in zebrafish microglia.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    mpeg1.1
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75023-plasmid-map-sequence-id-141914

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Showing: 10561 - 10590 of 169885 results