161,152 results

1. lentiCRISPR v2 sgKEAP1-3

   Plasmid

   #186837

   Purpose

   knock out KEAP1 in mammalian cells

   Depositor

   Boyi Gan

   Article

   Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

   Insert

   Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIA028

   Plasmid

   #200419

   Purpose

   thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning

   Depositor

   Rut Carballido-López

   Article

   Altinoglu et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Tags

   PAmCherry1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-luxKate

   Plasmid

   #229951

   Purpose

   Mammalian expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

   Depositor

   Andy Yeh

   Article

   Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

   Insert

   luxKate
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p212 pCMV-EGFP/RFP

   Plasmid

   #8389

   Purpose

   Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-smURFP-IRES-eGFP

   Plasmid

   #80343

   Purpose

   Expresses smURFP and eGFP in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   smURFP
   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLminP_Luc2P_RE60

   Plasmid

   #90405

   Purpose

   STAT5 - Stat5 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SIE

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1 gfaABC1D-BioID2-BioID2-HA

   Plasmid

   #176740

   Purpose

   Expresses the promiscuous small biotin ligase, BioID2, in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT Smarca4-sfGFP

    Plasmid

    #107056

    Purpose

    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CS2 Flag-Smad2

    Plasmid

    #14042

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETSUMO-TtAgo

    Plasmid

    #210191

    Purpose

    Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

    Depositor

    Phillip Zamore

    Article

    Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

    Insert

    TtAgo (TT_P0026 Thermus thermophilus)
    

    Use

    Tags

    6x His tag and SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. RAB11A-GFP HDRT Source (pTR 143)

    Plasmid

    #112012

    Purpose

    DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    RAB11A-GFP HDRT (RAB11A Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDB-hCRY1-mScarlet-I-CD4-bla

    Plasmid

    #179442

    Purpose

    Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    mScarletI
    

    Use

    CRISPR

    Tags

    His/Flag

    Expression

    Mutation

    Promoter

    None

    Available since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 NQO1-Flag

    Plasmid

    #61729

    Purpose

    Expresses human NQO1 Flag tagged at C-terminus

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al J Biol Chem. 2011 Mar 18;286(11):8839-45

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBa-LSS-GFP-LDLR wt

    Plasmid

    #98184

    Purpose

    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    GFP and LDLR signal sequence, N-term of GFP so GF…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta Actin

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21a-AspRS-His

    Plasmid

    #124106

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Aspartate-tRNA ligase (aspS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid

    #22028

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBAC-lacZ

    Plasmid

    #13422

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cas9-GFP_sg_mTfeb

    Plasmid

    #79006

    Purpose

    Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    sgTfeb (Tfeb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MSCV-myc-CAR-2A-Thy1.1

    Plasmid

    #127890

    Purpose

    Expresses CART receptor along with tags.

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Inserts

    myc,
    CAR
    2A
    Thy1.1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid

    #85165

    Purpose

    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

    Depositor

    Robin Dowell

    Article

    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

    Insert

    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    

    Use

    Tags

    AmilCP and RTX

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-ESR1

    Plasmid

    #65211

    Purpose

    Full length mammalian expression vector for hESR1

    Depositor

    Ratna Vadlamudi

    Insert

    esr1 (ESR1 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJ3 SHP1 WT

    Plasmid

    #8572

    Depositor

    Ben Neel

    Insert

    SHP1 (PTPN6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

30. RABGEF1A-c014

    Plasmid

    #175478

    Purpose

    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABGEF1 (RABGEF1 Human)
    

    Use

    Tags

    His6-Zbasic-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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