We narrowed to 23,288 results for: CRISPR

1. pCas28

   Plasmid

   #82385

   Purpose

   sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23108

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas27

   Plasmid

   #82384

   Purpose

   sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PJ23117

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK412

   Plasmid

   #155371

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MsMUT_sgRNA1

   Plasmid

   #111296

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA1 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MsMUT_sgRNA3

   Plasmid

   #111297

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA3 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHR-EF1a-dCas9-HA-BFP-KRAB-NLS

   Plasmid

   #102244

   Purpose

   Lentiviral expression of dCas9-HA-BFP-KRAB-NLS for CRISPRi driven by a hEF1alpha promoter

   Depositor

   Jacob Corn

   Insert

   dCas9-HA-BFP-KRAB-NLS
   

   Use

   CRISPR and Lentiviral

   Tags

   HA-TagBFP-KRAB-NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1a-PEmax-P2A-GFP

   Plasmid

   #202000

   Purpose

   Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

   Depositor

   Daniel Geschwind

   Insert

   PEmax-P2A-GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

   Plasmid

   #221385

   Purpose

   Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

   Depositor

   Junwei Shi

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX408 Francisella tularensis subsp. novicida Cas9

   Plasmid

   #68705

   Purpose

   Francisella tularensis subsp. novicida Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. miniTurbo-dCasRx

    Plasmid

    #219821

    Purpose

    EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCasRx
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Promoter

    EF1a and hU6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid

    #104413

    Purpose

    transient expression of dCas9-dTET1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dTET1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    H1672Y-D1674A

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid

    #68336

    Purpose

    Campylobacter lari CF89-12 Cas9

    Depositor

    Feng Zhang

    Insert

    ClCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Promoter

    SNR52p

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid

    #104418

    Purpose

    transient expression of dCas9-dJMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    dJMJD2A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    S288G-T289G

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX399 Mycoplasma gallisepticum str. F Cas9

    Plasmid

    #68335

    Purpose

    Mycoplasma gallisepticum str. F Cas9

    Depositor

    Feng Zhang

    Insert

    MgCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTFAP2B-donor

    Plasmid

    #113823

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFAP2B with GFP

    Depositor

    Kevin White

    Insert

    TFAP2B homology arms flanking EGFP-IRES-Neo cassette (TFAP2B Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pZBTB12-donor

    Plasmid

    #113802

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZBTB12 with GFP

    Depositor

    Kevin White

    Insert

    ZBTB12 homology arms flanking EGFP-IRES-Neo cassette (ZBTB12 Human)
    

    Use

    CRISPR

    Mutation

    rs9267664, rs9267663

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTSHZ2-donor

    Plasmid

    #113810

    Purpose

    CRISPR donor plasmid to tag human transcription factor TSHZ2 with GFP

    Depositor

    Kevin White

    Insert

    TSHZ2 homology arms flanking EGFP-IRES-Neo cassette (TSHZ2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXD1-donor

    Plasmid

    #112350

    Purpose

    CRISPR donor plasmid to tag human transcription factor MXD1 with GFP

    Depositor

    Kevin White

    Insert

    MXD1 homology arms flanking EGFP-IRES-Neo cassette (MXD1 Human)
    

    Use

    CRISPR

    Mutation

    hg38:2:69937537:A>C,rs3771530

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZNF423-donor

    Plasmid

    #113822

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZNF423 with GFP

    Depositor

    Kevin White

    Insert

    ZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge