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pRRL-GFPAdGO-PGK-Neo

Plasmid

#136898

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPAdGO

Use

Lentiviral

Tags

2xNLS

Mutation

TGG>TGA

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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TS181_pETNKI_his3C_pA-Dam

Plasmid

#200721

Purpose

producing pA-DamID methylation protein in bacteria

Depositor

Bas van Steensel

Article

van Schaik et al Methods Mol Biol. 2022;2458:215-229. doi

Insert

His3C-pA-Dam

Tags

His

Expression

Bacterial

Mutation

codon optimized

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pEcCas6

Plasmid

#170091

Purpose

expresses E. coli type I-E Cas6

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas6

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLBH545_Tet-Cas14a1_Locus

Plasmid

#112501

Purpose

Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCMT1_PCMT

Plasmid

#109932

Purpose

Protein expression and purification of PCMT1_PCMT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCMT1_PCMT

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCse2

Plasmid

#170094

Purpose

expresses E. coli type I-E Cse2

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cse2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLBH559_Tet-HisCas14a1Locus

Plasmid

#112502

Purpose

Expresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

Depositor

Jennifer Doudna

Article

Harrington et al Science. 2018 Oct 18. pii: science.aav42

Insert

Cas14a1

Use

CRISPR

Tags

6xHis-TEV

Expression

Bacterial

Promoter

Tet

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-ITGAM POD-nAb-WPRE

Plasmid

#244975

Purpose

Expresses a fusion protein of a nanobody against integrin alpha M (ITGAM; CD11b) and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Article

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

ITGAM POD-nAb

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA+WT GADD45

Plasmid

#24929

Depositor

Xin Wang

Article

Yang et al J Biol Chem. 2000 Nov 24. 275(47):36892-

Insert

GADD45 (GADD45A Human)

Tags

HA

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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hNET pcDNA3

Plasmid

#15475

Depositor

Randy Blakely

Article

Galli et al J Exp Biol. 1995 Oct . 198(Pt 10):2197-2

Insert

hNET (SLC6A2 Human)

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pJAT10

Plasmid

#204293

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pBM-strep-UCP1

Plasmid

#216207

Purpose

for expression of human strep-tagged UCP1 protein in BacMam system

Depositor

Lei Chen

Article

Kang et al Nature. 2023 Aug;620(7972):226-231. doi:

Insert

UCP1 (UCP1 Human)

Tags

strep

Expression

Mammalian

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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Keratin 18 (pcDNA3)

Plasmid

#18064

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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