We narrowed to 11,499 results for: ARIA
-
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F2shRNA human
Plasmid#66884PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F2DepositorAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF8-N-terminus(1-111)
Plasmid#64671PurposeExpresses only N terminus of RNF8, amino acids 1 to 111DepositorInsertring finger protein 8 (RNF8 Human)
TagsGFPExpressionMammalianMutationcontaining only N-terminus amino acids 1 to 111PromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
R
Plasmid#167170PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cellsDepositorInsertsATP1A1-R
ATP1B1
ExpressionBacterial and InsectPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.DS-SOSIP.4mut-gp160
Plasmid#123277PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variantDepositorInsertHIV-1 (BG505) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N
Plasmid#113952Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S and S212N su…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.DS-SOSIP.6mut-gp160
Plasmid#123278PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variantDepositorInsertHIV-1 (BG505) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)
Plasmid#114102Purposefusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGLucM23-iChloC-EYFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 FS10
Plasmid#113956Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitution…PromotercmvAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNot4-GB1His6_AG
Plasmid#148792PurposeBacterial Expression of HsNot4DepositorInsertHsNot4 (CNOT4 Human)
ExpressionBacterialMutationone silent mutation compared to the sequence give…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RNF8-C-terminus
Plasmid#64673Purposeexpresses only C terminus of RNF8, amino acids 402-485DepositorInsertRing finger protein 8 (RNF8 Human)
TagsGFPExpressionMammalianMutationcontaining only C-terminus amino acids 402 to 485PromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Prom1 D-5
Plasmid#184017PurposeMammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.DepositorInsertProm1 SV8(-Ex19a) D-5 (Prom1 Mouse)
TagsFlagExpressionMammalianMutationDeletion of exon 19a (amino acids 696-701), and d…Available SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTER E2F3shRNA human
Plasmid#66885PurposeDoxycycline-regulated mammalian expression vector for expressing shRNA against E2F3DepositorAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-CheRiff-EGFP
Plasmid#114108Purposefusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
UseAAVExpressionMammalianPromoterhSynAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG426_UV5
Plasmid#165608PurposeVector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)DepositorInsertlacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialMutationD1135V, S1136R, D1332K and R1333G mutations in Sp…PromoterlacUV5 driving Cas9 VRKG and UV5 driving sgRNAAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-N-gamma-tubulin (1-333)
Plasmid#87858PurposeFluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLSDepositorInsertgamma-tubulin 1 (TUBG1 Human)
TagsGFPExpressionMammalianMutationFragment from aa 1 to 333Available SinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-RNF8-N-terminus(1-140)
Plasmid#64675Purposeexpresses only N terminus of RNF8, amino acids 1 to 140DepositorInsertRing finger protein 8 (RNF8 Human)
TagsFLAGExpressionMammalianMutationonly N terminus amino acids 1-140PromoterCMVAvailable SinceMay 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFB-Puro-A
Plasmid#192506PurposeFor cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a nd a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.DepositorInserthU6-sgRNA-CS1-BsmBI-EFS-Puro-WPRE
UseLentiviralPromoterhU6Available SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 a22-end_S212N
Plasmid#113951Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, S212N substitutionPromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFB-Puro-B
Plasmid#192507PurposeFor cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.DepositorInsertbU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE
UseLentiviralPromoterbU6Available SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-DC-mutant-G435R
Plasmid#64684Purposeexpresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patientsDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
TagsGFPExpressionMammalianMutationmutation at G435RPromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-DC-mutant-R398W
Plasmid#64683Purposeexpresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patientsDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
TagsGFPExpressionMammalianMutationmutation at R398WPromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-hB90
Plasmid#18968DepositorInsertBrevican C-terminus (BCAN Human)
ExpressionMammalianMutationHuman brevican signal peptide aa 1-23 followed by…Available SinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-DC-mutant-F164L
Plasmid#64681Purposeexpresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patientsDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
TagsGFPExpressionMammalianMutationmutation at F164LPromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-DC-mutant-H376Y
Plasmid#64682Purposeexpresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patientsDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
TagsGFPExpressionMammalianMutationmutation at H376YPromoterCMVAvailable SinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
S
Plasmid#167172PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cellsDepositorInsertsATP1A1-S
ATP1B1
ExpressionBacterial and InsectPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Major Sat-BUB1
Plasmid#133350PurposeExpress a TALE construct that recognizes major satellite repeats fused to mClover and the kinase domain of mouse BUB1 (aa 672-1058) at the C terminusDepositorAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only