We narrowed to 10,431 results for: plasmids 101

1. pSI-Check2-hRluc-NFkB-firefly

   Plasmid

   #106979

   Purpose

   Luciferase reporter with internal control for NFkB signal.

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   NFKB recognition sites (NFKB1 Human)
   

   Use

   Luciferase

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti_EFS-Cas9-P2A-HygR

   Plasmid

   #164134

   Purpose

   Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRGLO-GLIS2-201

   Plasmid

   #117331

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR GLIS2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. tetO-MITF

   Plasmid

   #170695

   Purpose

   doxycycline-inducible overexpression of MITF

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   tight TRE promoter

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. gh69

   Plasmid

   #106740

   Purpose

   expression of gRNA targeting B3GNT3

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B3GNT3 (B3GNT3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh262

   Plasmid

   #106853

   Purpose

   expression of gRNA targeting ALG9

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ALG9 (ALG9 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIX403_UBAP2L_mCherry

    Plasmid

    #105286

    Purpose

    puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    UBAP2L (UBAP2L Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-U6/sgClta-sfGFP-Clta

    Plasmid

    #200261

    Purpose

    U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

    Depositor

    Justin Eyquem

    Article

    Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

    Insert

    sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
    

    Use

    AAV

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

    Plasmid

    #225355

    Purpose

    Lentiviral vector for ActivinA and BFP expression

    Depositor

    Wendell Lim

    Article

    Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

    Insert

    Actvin A and TagBFP (Inhba Mouse, Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGEN-Dre-nls (JDW 1131)

    Plasmid

    #229809

    Purpose

    A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    DRE recombinase-nls
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUW-tetO-EGFP

    Plasmid

    #84041

    Purpose

    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pME Actin Vhh mCherry (JDW 1240)

    Plasmid

    #224499

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Actin-Vhh-mCherry
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-DreERT2 (JDW 1344)

    Plasmid

    #224523

    Purpose

    A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    DreERT2
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Puro CAG eGFP NLS

    Plasmid

    #170830

    Purpose

    Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. rag2:mcherry_CE_pISceI

    Plasmid

    #223047

    Purpose

    Express m-Cherry gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    m-Cherry
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL3 Zscan4 promoter

    Plasmid

    #69249

    Purpose

    Luciferase reporter for Dux4 activation of Zscan4 transcription

    Depositor

    Stephen Tapscott

    Article

    Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:

    Insert

    Zscan4 (ZSCAN4 Human)
    

    Use

    Luciferase

    Mutation

    The two single nucleotide mismatches found betwee…

    Promoter

    Zscan4

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti_CMV-Cas9-P2A-HygR

    Plasmid

    #164133

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by CMV promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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