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TEN

Plasmid

#12498

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

TEN

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Available Since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pLIC.B4.BSU37660

Plasmid

#11535

Depositor

Sung-Hou Kim

Article

Xu et al J Struct Funct Genomics. 2005 Dec;6(4):2

Insert

phosphotransacetylase

Tags

HisTag-MBP, TEV cleavable

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pASUS17R

Plasmid

#1205

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed ser 17 to arg

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pPS1701

Plasmid

#8852

Depositor

Pamela Silver

Article

Kahana et al Mol Biol Cell. 1998 Jul . 9(7):1741-56.

Insert

NIP100 (NIP100 Budding Yeast)

Tags

3x HA

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pDD2045

Plasmid

#101379

Purpose

Name: pBluescript-empty vector. pBluescript-LANApi,K14 (pDD2000) bidirectional empty vector for backbone only.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Type

Empty backbone

Use

Unspecified

Availability

Academic Institutions and Nonprofits only

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pT4/HB

Plasmid

#108352

Purpose

Optimized Sleeping Beauty Transposon Vector with MCS

Depositor

Wolfgang Uckert

Article

Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

Type

Empty backbone

Use

Transposon

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-Traditional CAR (anti-CD19)

Plasmid

#192115

Purpose

Lentiviral delivery of Traditional CAR(anti-CD19)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

Plasmid

#175539

Purpose

Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Cox8a-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)

Plasmid

#194728

Purpose

AAVS1-tdTomato targeting vector, compartible with Cpf1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

tdTomato

Use

CRISPR; Knockin targeting plasmid

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-HiBiT

Plasmid

#201489

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HiBiT

Tags

HiBit

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag9

Plasmid

#175545

Purpose

Expression of HaloTag9 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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