We narrowed to 8,680 results for: sgRNA

1. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Expression

   Mammalian

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-Solo-mScarlet-H2BC11

   Plasmid

   #207756

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. B270 + SPRTN sgSTOP

   Plasmid

   #100718

   Purpose

   B270 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SPRTN (cloned using BbsI)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. c3GIC9n

   Plasmid

   #62192

   Purpose

   col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9n (D10A variant) expression. Contains NsiI cloning site for U6-sgRNA cassettes

   Depositor

   Lukas Dow

   Article

   Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

   Insert

   humanized S. Pyogenes Cas9 (D10A)
   

   Use

   CRISPR

   Tags

   HA

   Mutation

   D10A substitution (Cas9n)

   Promoter

   TRE3G

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-EN1082_Rad21_STOP

   Plasmid

   #156450

   Purpose

   For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   Cas9, RAD21 (Rad21 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdTomato/pTREX-b

   Plasmid

   #68709

   Purpose

   Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

   Depositor

   Roberto Docampo

   Article

   Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

   Insert

   tdTomato
   

   Use

   Expression of tdtomato red fluorescence protein i…

   Available Since

   Oct. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pJG85

   Plasmid

   #89281

   Purpose

   Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

   Depositor

   Daniel Voytas

   Article

   Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK615

   Plasmid

   #110609

   Purpose

   BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   sgRNA
   

   Use

   Synthetic Biology

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-U6-pegRNA_SRD5A3-EGFP

   Plasmid

   #159781

   Purpose

   S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CSAC-Crys

    Plasmid

    #164967

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    hU6, hSyn

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ141-ZmUbi-RZ-Aac

    Plasmid

    #129685

    Purpose

    (Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    Empty backbone
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CSAC-Bmal1

    Plasmid

    #168296

    Purpose

    Expresses sgRNA in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgBmal1-1-hU6-sgBmal1-3
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    U6, hSyn

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-evoA1-seBEN_empty

    Plasmid

    #174701

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEN
    

    Use

    Lentiviral

    Tags

    myc tag

    Promoter

    EFS/U6

    Available Since

    Nov. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK614

    Plasmid

    #110608

    Purpose

    BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC-Z-SpCas9-gRNA-repeat-polyT-11

    Plasmid

    #197781

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    gRNA repeat for U6 sgRNA assembly
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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