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CL20_mEGFP_TCF3_PBX1

Plasmid

#205937

Purpose

Express mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TCF3_PBX1 (TCF3, PBX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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NylCA-TS_pET28a

Plasmid

#215419

Purpose

Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylCA-TS

Expression

Bacterial

Mutation

S111G/A137L/E263Q

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRF0G-Tyr

Plasmid

#113644

Purpose

Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

Depositor

Jeffrey Barrick

Article

Monk et al ACS Synth Biol. 2016 Sep 20.

Insert

MjYRS and tRNA gene

Use

Synthetic Biology

Available Since

July 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pC081: pAAV.CMV.mCherry

Plasmid

#205744

Purpose

AAV vector for expressing CMV-driven mCherry reporter gene.

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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VE-cad KI gRNA1

Plasmid

#92310

Purpose

CRISPR-GFP-gRNA for cutting VEcad

Depositor

Sean Palecek

Article

Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

Insert

VEcad KI gRNA1 (CDH5 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PAX7_FOXO1

Plasmid

#205891

Purpose

Express mEGFP-tagged fusion protein, PAX7_FOXO1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PAX7_FOXO1 (PAX7, FOXO1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR CD22-Megf10 CAR

Plasmid

#113016

Purpose

for production of lentivirus encoding CD22 CAR-P Megf10

Depositor

Ron Vale

Article

Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

Insert

CD22 CAR-P Megf10 intracellular domain

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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phU1-PspCas13b-non-targeting (pEZ36)

Plasmid

#229053

Purpose

Cloning backbone for PspCas13b crRNA (Human U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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mc2 neo EF1a

Plasmid

#206220

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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