165,567 results

1. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

   Plasmid

   #220611

   Purpose

   Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-3x HA-hM3D(Gq)
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-neo (1795)

   Plasmid

   #41000

   Depositor

   Jonathon Pines

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmBanana-Hyg-CTCF-CtermNES-N1

   Plasmid

   #131851

   Purpose

   Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   CTCF (CTCF Human)
   

   Tags

   mBanana

   Expression

   Mammalian

   Mutation

   the C-terminus of CTCF is linked to HIV-Rev prote…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK18sB

   Plasmid

   #177838

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-hIFITM1

   Plasmid

   #58399

   Purpose

   Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

   Depositor

   Howard Hang, Jacob Yount

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM1 (IFITM1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-pur H-2Kb

   Plasmid

   #111623

   Purpose

   Sleeping Beauty transposon vector encoding H-2Kb mouse MHC Class I allele

   Depositor

   Jon Yewdell

   Insert

   H2Kb
   

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_EGFP

   Plasmid

   #25899

   Purpose

   Gateway entry vector containing EGFP.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Cristina Montagna, Elias Spiliotis

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

    Plasmid

    #169930

    Purpose

    Overexpression of 1-270AA CycK-GFP fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Insert

    CCNK (CCNK Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-mROCK2

    Plasmid

    #101296

    Purpose

    Mammalian expression of Rock2-GFP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock2 (Rock2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

    Plasmid

    #156436

    Purpose

    For transient expression of human PDS5B tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    human PDS5B (PDS5B Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_EML4_ALK

    Plasmid

    #205809

    Purpose

    Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EML4_ALK (EML4, ALK Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMET7-GAG-EGFP

    Plasmid

    #80605

    Purpose

    Mammalian expression vector that expresses HIV-1 GAG with EGFP fused to it

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-PBX1

    Plasmid

    #170699

    Purpose

    doxycycline-inducible overexpression of PBX1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    PBX1 (PBX1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pON.mCherry

    Plasmid

    #84821

    Purpose

    Constitutive Expression plasmid with mCherry fluorescent protein

    Depositor

    Howard Shuman

    Article

    Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

    Insert

    mCherry
    

    Expression

    Bacterial

    Promoter

    Tac Promoter

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE38

    Plasmid

    #90378

    Purpose

    RELB - NFkB gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    MAF

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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