We narrowed to 13,797 results for: FLU

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pTD-NTwinStrep_Sm

Plasmid

#45937

Purpose

use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Plasmid

#187079

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pACUH-GFP1-10 mitochondrial matrix

Plasmid

#172063

Purpose

UAS vector to express GFP1-10 in the mitochondrial matrix

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP1-10 mitochondrial matrix

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFX-β-tubulin-mCherry

Plasmid

#175829

Purpose

Express mCherry with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR

Plasmid

#118468

Purpose

cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19/ER-mChe

Plasmid

#183163

Purpose

Construct for transient expression of endoplasmic reticulum-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

ER-mCherry

Tags

25 aa ER signal of Glycine soja kunitz-type tryps…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFnCpf1GG

Plasmid

#131010

Purpose

Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of FnCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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hHS1

Plasmid

#159169

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Article

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

hHS1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUC19/NLS-mChe

Plasmid

#183162

Purpose

Construct for transient expression of cell nucleus-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

NLS-mCherry

Tags

16 aa nuclear localization signal and 16 aa nucl…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-B7-EYFP

Plasmid

#114110

Purpose

fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

Inserts

Gaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein

Use

AAV

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138129

Purpose

Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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mRuby2-ZO1-C-14

Plasmid

#55916

Purpose

Localization: Tight Junctions, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

ZO1 (TJP1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTagBFP2-Annexin-12

Plasmid

#55276

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Annexin (A4) (ANXA4 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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ZJOM119

Plasmid

#133661

Purpose

Integration of NAB2-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-mTagBFP2 (NAB2 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-AKARev

Plasmid

#118478

Purpose

ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-AKARev

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEB1-moxCerulean3

Plasmid

#103975

Purpose

Plasmid encoding moxCerulean3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxCerulean3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pR-mTagBFP2

Plasmid

#228596

Purpose

Mammalian expression of mTagBFP2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTagBFP2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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