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p7127 pHAGE-P-CMVt-N-HA-GAW-FTL

Plasmid

#100153

Purpose

Expresses FTL

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

FTL (FTL Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBPJ_WT

Plasmid

#82158

Purpose

Gateway Donor vector containing RBPJ , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBPJ (RBPJ Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Galectin3-CD4d3+4-bio

Plasmid

#73128

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

LGALS3 (LGALS3 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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JAM1-CD4d3+4-bio

Plasmid

#73110

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding JAM1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

JAM1 (F11R Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-mouseAtoh1-IRES-GFP

Plasmid

#73572

Purpose

Mammalian retroviral expression of Atoh1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Promoter

MSCV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
His-TEV-Aurein1.2_(+36GFP)-LPETG-His

Plasmid

#71755

Purpose

His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

Aurein1.2_(pos36_GFP)-LPETG-His

Tags

6XHis, Sortase (LPETG), and TEV Protease

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-FHM

Plasmid

#51871

Purpose

Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

Depositor

Kenshi Hayashi, Tomoko Tahira

Article

Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

Insert

MIBP1 (Hivep2 Rat)

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHH

Plasmid

#51057

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pIC242

Plasmid

#44432

Depositor

Iain Cheeseman, Arshad Desai

Article

Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Insert

Mob1A (MOB1A Human)

Use

Retroviral

Tags

GFP, s peptide, and tev

Expression

Mammalian

Mutation

F5L, K15R and G212R

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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pGFH14A5

Plasmid

#39542

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Amino acids 96-100 changed to alanine

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pIOM14

Plasmid

#20868

Depositor

Carmen Beuzón

Article

Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

Insert

aphA::aacC1

Expression

Bacterial

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only
pHS1993

Plasmid

#239836

Purpose

OMEGAOff-KRK mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

DNMT3A-DNMT3L-OrufIscB-KRK-KRAB

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1 + E137K/E4…

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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U6-PGK-hCD2

Plasmid

#224294

Purpose

Expresses sgRNA from U6 promoter and human CD2 under PGK

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

U6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6/PGK

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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CD55-full length

Plasmid

#220874

Purpose

Expresses CD55 variant in mammalian cells

Depositor

Ferenc Scheeren

Insert

CD55 (CD55 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMW

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PX459-CDKN1A

Plasmid

#217457

Purpose

For CDKN1A (p21) knockout

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CRISPR Cas9 guide for CDKN1A (CDKN1A Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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HSPB1

Plasmid

#155670

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

HSPB1 (HSPB1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

Plasmid

#171587

Purpose

Bacterial expression of NLS-RfxCas13d-NLS 6His

Depositor

Neville Sanjana

Article

Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

Insert

MKIEE-NLS-RfxCas13d-NLS-HA-6His

Tags

HA-6HIS and MKIEE

Expression

Bacterial

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-IpgD(C439S)

Plasmid

#183667

Purpose

N-terminally tagged Shigella flexneri IpgD (catalytically inactive) for mammalian expression

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Tags

EGFP

Expression

Mammalian

Mutation

C439S

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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