We narrowed to 63,682 results for: cell

1. mCherry-7aa-ActA-pEGFP-C1

   Plasmid

   #177406

   Purpose

   To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

   Depositor

   David Andrews

   Article

   Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. attB53-Pac-TRE-3xFlag-Neurog1-attB53

   Plasmid

   #183613

   Purpose

   RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   Neurog1 (Neurog1 Mouse)
   

   Use

   Rmce

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

   Plasmid

   #187358

   Purpose

   Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   HPC-4 antibody heavy chain, and 6xHIS tag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3XFlag pCMV5-TOPO TAZ (106∂)

   Plasmid

   #24814

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of aa 1-106

   Promoter

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pLXSN-H-Ras_N17

   Plasmid

   #39537

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

   Insert

   HRAS N17 (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N17

   Promoter

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

   Plasmid

   #154775

   Purpose

   plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAA in GFP reporter, UUA anticodon and G10A, …

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pssGFP-hCRT

   Plasmid

   #80022

   Purpose

   Mammalian expression of signal peptide-EGFP-humanCRT

   Depositor

   Chinten James Lim

   Article

   Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

   Insert

   CRT (CALR Human)
   

   Use

   Tags

   EGFP and signal peptide

   Expression

   Mammalian

   Mutation

   signal peptide 17aa added before GFP-CRT, will ta…

   Promoter

   CMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. WDR5-HaloTag

   Plasmid

   #200881

   Purpose

   Mammalian expression of human WDR5 with C-terminal HaloTag fusion

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

   Insert

   WDR5-HaloTag (WDR5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 FLAG DEPTOR (PDZ domain)

   Plasmid

   #21701

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

   Insert

   DEPTOR PDZ domain (DEPTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   PDZ domain only

   Promoter

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGP18 (CK2alpha' K69M)

    Plasmid

    #27090

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha' K69M (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K69M

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pSLIK S6K (E389-deltaCT) neo

    Plasmid

    #58516

    Purpose

    Lentiviral expression vector for an inducible HA-tagged rat S6K1-E389-deltaCT

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Rps6kb1 (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pENTR FOXP3

    Plasmid

    #16363

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

13. KIF5C(1-560)-2xmCitrine-GFP11

    Plasmid

    #61667

    Purpose

    Tandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-sh-mSlug-3

    Plasmid

    #40647

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-3 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pRK5 FLAG DEPTOR (DEP domains)

    Plasmid

    #21700

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR DEP domains (DEPTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    DEP domains only

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA

    Plasmid

    #176458

    Purpose

    Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoter

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    humanized S. pyogenes Cas9-XTEN-Ec73RT fusion
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Budding Yeast, Human, Synthetic)
    

    Use

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

    Plasmid

    #139649

    Purpose

    Reporter assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    H2B-mCherry
    

    Use

    Lentiviral

    Tags

    FLAG, His6

    Expression

    Mutation

    Promoter

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-HBP1

    Plasmid

    #16178

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    HMG-box transcription factor 1 (HBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

20. px458_2A_GFP_sgRNA_EIF2AK2

    Plasmid

    #106108

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting EIF2AK2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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