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pKDR-EF1a.10

Plasmid

#183573

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 10

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.11

Plasmid

#183574

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 11

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.12

Plasmid

#183575

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 12

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.5

Plasmid

#183572

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 5

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.3

Plasmid

#183570

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 3

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a.1

Plasmid

#183568

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 1

Expression

Mammalian

Promoter

EF1a

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pKDR-CBh.21

Plasmid

#183565

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Attenuator Sequence 21

Expression

Mammalian

Promoter

CBh

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S19-T48C

Plasmid

#128597

Purpose

Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S19 (rpsS E.coli)

Expression

Bacterial

Mutation

T48C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S15-T79C

Plasmid

#133048

Purpose

Expresses tagless S15-T79C mutant ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S15 E.coli ribosomal protein (rpsO E.coli)

Expression

Bacterial

Mutation

T79C

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S7-S83C/Δ157-178

Plasmid

#128592

Purpose

Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S7 E.coli ribosomal protein (rpsG E.coli)

Expression

Bacterial

Mutation

S83C, Δ157-178

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0130

Plasmid

#172809

Purpose

Level 1 module containing 35S-CTP-mCHERRY-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0170

Plasmid

#172813

Purpose

Level 1 module containing nCFP_P4 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-SP_SV40-CFP-3U+TERNOS

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0150

Plasmid

#172811

Purpose

Level 1 module containing 35S-CTP-mCHERRY-cVENUS_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-3XFLAGcVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0140

Plasmid

#172810

Purpose

Level 1 module containing 35S-CTP-mCHERRY-nVENUS_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-nVENUS-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0120

Plasmid

#172808

Purpose

Level 1 module containing 35S-CTP-mCHERRY-nYFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mCHERRY-3XFLAGnYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0050

Plasmid

#172801

Purpose

Level 1 module containing 35S-HSP21-cYFP_P3 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-cYFP3XHA-3U+TER35S

Use

Synthetic Biology

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0080

Plasmid

#172786

Purpose

Level 0 module containing NT2_nVenus

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBC0131

Plasmid

#172793

Purpose

Level 0 module containing CDS2ns_deltamPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 with C-ter delection (41-252), Arabidopsis thaliana

Use

Synthetic Biology

Mutation

no

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLBT0010

Plasmid

#172797

Purpose

Level 1 module containing 35S-HSP21-GFP_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-NT2_CTPSSU-mHSP21-GFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBT0020

Plasmid

#172798

Purpose

Level 1 module containing 35S-HSP21-nYFP_notag_P2 F

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

p35S-5UTMV-CTPSSU-mHSP21-nYFP-3U+TER35S

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge