19,660 results

1. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

   Plasmid

   #52093

   Purpose

   Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

   Inserts

   CD3 delta (Cd3d Mouse)
   CDC gamma (Cd3g Mouse)
   CD3 epsilon (Cd3e Mouse)
   CD3 zeta (Cd247 Mouse)
   

   Use

   Retroviral

   Tags

   F2A, IRES-EGFP, P2A, and T2A

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLPCX-VEcadTL

   Plasmid

   #45849

   Depositor

   Martin Schwartz

   Article

   Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

   Insert

   VE-cadherin (Cdh5 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

   Plasmid

   #50458

   Purpose

   Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Has Service

   AAV Retrograde and AAV8

   Insert

   rM3D-mCherry
   

   Use

   AAV

   Tags

   HA and mCherry

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. BIC-Gag-CAS9

   Plasmid

   #119942

   Purpose

   encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of GAG-CAS9 Virus like particles from producer cells in association with over expressed gRNA(s) and appropriate envelopes.

   Depositor

   Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG FMLV fused to spCAS9
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   hCMV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHO4d-Cas9

   Plasmid

   #67881

   Purpose

   Cas9nlsHis6 Bacterial Expression construct

   Depositor

   Michael Nonet

   Article

   Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and T7 epitope

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-OTUD3 (OTU, aa 52-209)

   Plasmid

   #61412

   Purpose

   Expresses human OTUD3 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD3 (OTUD3 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-51 and aa 210-398.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQCXIN-TetR-mCherry

   Plasmid

   #59417

   Purpose

   retroviral construct expressing the Tet repressor (TetR) tagged with mCherry

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Tet repressor protein (TetR)
   

   Use

   Retroviral

   Tags

   mCherry

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. myc-mULK1

    Plasmid

    #31960

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    ULK1 (Ulk1 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pCSCMV:tdTomato

    Plasmid

    #30530

    Purpose

    mammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.

    Depositor

    Gerhart Ryffel

    Article

    Waldner et al BMC Dev Biol. 2009 Jun 23;9:37. doi: 10.

    Has Service

    Cloning Grade DNA

    Insert

    tdTomato
    

    Expression

    Mammalian

    Mutation

    R415H

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-dCas9-dMQ1-EGFP

    Plasmid

    #89637

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR

    Tags

    3*FLAG, 6*His, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

    Plasmid

    #81251

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCer-C3-Plk1-K82R

    Plasmid

    #68134

    Purpose

    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    K82R kinase-dead version

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

    Plasmid

    #114070

    Purpose

    T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

    Plasmid

    #175573

    Purpose

    Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-MMLV_RT (mut H)

    Plasmid

    #165546

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA-SADB19L

    Plasmid

    #32632

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19L
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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