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pSpp1-is2

Plasmid

#58248

Purpose

Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1-is2 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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Arch(D95H)-eGFP

Plasmid

#51081

Purpose

Expresses Arch(D95H)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage

Depositor

Adam Cohen

Article

Park et al PLoS One. 2013 Dec 31;8(12):e85221. doi:

Insert

Archaerhodopsin-3 w/ D95H point mutation fused to eGFP

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Changed Aspartic Acid 95 to Histidine

Promoter

Ubiquitin

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

Plasmid

#24549

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Available Since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pGWB416

Plasmid

#74810

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB507

Plasmid

#74849

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

Plasmid

#133282

Purpose

Lentiviral expression of a peroxisomal eGFP

Depositor

Etienne Sokal

Article

Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

Insert

Peroxisome targeting signal 1

Use

Lentiviral

Tags

Peroxisome targeting signal 1

Expression

Mammalian

Promoter

None

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-MinE-His

Plasmid

#133623

Purpose

Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

Depositor

Petra Schwille

Article

Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

Insert

MinE-His (minE E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB423

Plasmid

#74817

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Human, Neurospora crassa, Synthetic)

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Promoter

No promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pVoPo-mSc

Plasmid

#187842

Purpose

Constitutive mScarlet-I-expression vector for Fusobacterium nucleatum

Depositor

Jörg Vogel

Article

Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

Insert

mScarlet-I

Expression

Bacterial

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJZC504

Plasmid

#62280

Purpose

PCP-VP64 fusion

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

PCP-VP64

Tags

VP64

Expression

Yeast

Promoter

pAdh

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET mOCR His6 LIC cloning vector (2Oc-T)

Plasmid

#37238

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and mOCR

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Expression

Plant

Promoter

Ubi

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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P823_eSpCas9(1.1)-RecA

Plasmid

#87264

Purpose

Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Tags

FLAG

Expression

Mammalian

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-2

Plasmid

#67017

Purpose

G protein beta-2 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

NM_005273.3 (GNB2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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LLP249 pGK-dCas9-DNMT3a V3

Plasmid

#100938

Purpose

dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)

Use

CRISPR and Lentiviral

Tags

3xHA and 3xTy1

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_NTRK3

Plasmid

#205817

Purpose

Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_NTRK3 (ETV6, NTRK3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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