We narrowed to 19,084 results for: iNOS

1. pcDNA3/td5StayGold(c4)=GianCreg

   Plasmid

   #212022

   Purpose

   For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GianCreg (GOLGB1 Human)
   

   Use

   Tags

   td5StayGold(c4)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Sac-Abeta(M1-42)

   Plasmid

   #71875

   Purpose

   Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

   Depositor

   Dominic Walsh

   Article

   Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

   Insert

   Abeta(M1-42) (APP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-Coff/Fon-mCherry

   Plasmid

   #137134

   Purpose

   Intersectional viral expression of mCherry in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_SFFv_Her2-BFP_RHL027

   Plasmid

   #164823

   Purpose

   Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKI_ΩBBMVP16&SRDXWUSm1

   Plasmid

   #220349

   Purpose

   Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   Superman Repression Domain X (SRDX) and VP16 tran…

   Expression

   Plant

   Mutation

   two amino acid substitution in WUS-box: L255A L25…

   Promoter

   CaMV 35S and RPS5A

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRIPZ-bioGEF-UBwt-PURO

   Plasmid

   #208045

   Purpose

   Enables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   Ub (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

   Plasmid

   #208048

   Purpose

   Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   BirAopt
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   BirAopt is human-codon-optimized BirA (PMID 15707…

   Promoter

   EFS

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-UltraCas12a-6xNLS

   Plasmid

   #209023

   Purpose

   Lentiviral vector expressing human codon-optimized UltraCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   UltraCas12a-6xNLS-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   M537R/F870L

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-HA-CDK4

    Plasmid

    #172612

    Purpose

    Expresses HA-tagged CDK4 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

    Plasmid

    #212968

    Purpose

    AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mNG,
    ωRNA*
    

    Use

    AAV and CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, T7 and U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-FBXL11

    Plasmid

    #126543

    Purpose

    Expresses GFP-tagged FBXL11 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    FBXL11 (KDM2A Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-Con/Fon-oScarlet

    Plasmid

    #137136

    Purpose

    Intersectional viral expression of oScarlet in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-oScarlet
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid

    #208942

    Purpose

    The CE1 construct, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ISDra2-TnpB-mCherry-U6-ωRNA*

    Plasmid

    #212967

    Purpose

    Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mCherry
    ωRNA*
    

    Use

    CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, T7 and U6

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV pmSyn1-EBFP-Cre

    Plasmid

    #51507

    Purpose

    Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Has Service

    AAV5

    Insert

    EBFP-Cre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    pmSyn1

    Available Since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-Con/Fon-mCherry

    Plasmid

    #137132

    Purpose

    Intersectional viral expression of mCherry in cells expressing both Cre AND Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Fon-mCherry
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 puro CXCR4 siRNA-2

    Plasmid

    #12272

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    CXCR4 siRNA (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2241

    Plasmid

    #111896

    Purpose

    ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple320 (POU4F1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple320 (POU4F1 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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