We narrowed to 9,404 results for: pho;

1. His-ATG3-H266A

   Plasmid

   #202784

   Purpose

   ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

   Depositor

   James Hurley

   Article

   Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

   Insert

   ATG3 (ATG3 Human)
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   H266A

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LENTICRISPR-PFKM_sgRNA1

   Plasmid

   #201610

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PFKM (PFKM Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LENTICRISPR-PFKM_sgRNA2

   Plasmid

   #201611

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   PFKM (PFKM Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LENTICRISPR-SNRNP70_sgRNA1

   Plasmid

   #201620

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SNRNP70 (SNRNP70 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. LENTICRISPR-SNRNP70_sgRNA2

   Plasmid

   #201621

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SNRNP70 (SNRNP70 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LENTICRISPR-NDUFB5_sgRNA1

   Plasmid

   #201606

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   NDUFB5 (NDUFB5 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-NDUFB5_sgRNA2

   Plasmid

   #201607

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   NDUFB5 (NDUFB5 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-Myc-PSK2 (K57A)

   Plasmid

   #197118

   Purpose

   Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

   Insert

   TAOK1 (K57A) (TAOK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Changed K 57 to A

   Promoter

   SV40

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-15b(+) Danio rerio PRL (Δprenylation motif)

   Plasmid

   #166417

   Purpose

   Express pET-15b(+) Danio rerio PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Danio rerio PRL (ptp4a3a Zebrafish)
   

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. VHACg/pDONR221

    Plasmid

    #172094

    Purpose

    Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221

    Depositor

    George Haughn

    Article

    Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

    Insert

    VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)
    

    Use

    Gateway entry vector

    Promoter

    530bp upstream of VHA-C ATG

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153542

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α1.1

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

    Plasmid

    #153541

    Purpose

    AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    EF1α1.1

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSUMO FAPP2-GLTPH

    Plasmid

    #170739

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    PLEKHA8-GLTPH (PLEKHA8 Human)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUMO ACD11

    Plasmid

    #170738

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

    Insert

    acd11 (ACD11 Mustard Weed)
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS405 SEC66-VAC8-GFP

    Plasmid

    #166840

    Purpose

    Expresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, SEC66 Budding Yeast)
    

    Tags

    GFP and SEC66 transmembrane domain

    Expression

    Yeast

    Mutation

    SEC66 transmembrane domain

    Promoter

    VAC8 promoter

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. LR (hsp70I: cPLA2-mK2)

    Plasmid

    #162564

    Purpose

    Measuring cPLA2 activation in live cells after heat shock.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    Heat shock protein 70 (HSPA1A Human)
    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    mK2

    Promoter

    cmlc2

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. MEN2A-MAC

    Plasmid

    #139634

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    MEN2A (RET Human)
    

    Tags

    StrepIII-HA-BirA*

    Expression

    Mammalian

    Mutation

    C634W

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GRB2-SHN

    Plasmid

    #139625

    Purpose

    StrepIII-HA-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Tags

    StrepIII-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FUG-T2A-H93R

    Plasmid

    #140739

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation H93R and GFP as two separate proteins

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-T2A-PTENH93R (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    H93R

    Promoter

    h

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FUG-PtenW274L

    Plasmid

    #140736

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation W274L as a GFP-PTEN fusion protein

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    W274L

    Promoter

    hUbiC

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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