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We narrowed to 5,023 results for: cre encoding plasmid

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Showing: 1401 - 1440 of 5023 results

  1. pTS2326

    Plasmid
    #169628
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    Sept. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    169628-plasmid-map-sequence-id-372400

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  2. pTS2317

    Plasmid
    #169624
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169624-plasmid-map-sequence-id-365608

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  9. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

    Plasmid
    #98932
    Purpose
    Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98932-plasmid-map-sequence-id-181244

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  11. pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40

    Plasmid
    #98931
    Purpose
    Cre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1 and AAV5
    Insert
    iGluSnFr
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98931-plasmid-map-sequence-id-190085

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  13. NXPE1_pcDNA3.4-TOPO

    Plasmid
    #226841
    Purpose
    Expresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.
    Depositor
    Bert Vogelstein
    Insert
    NXPE1 (NXPE1 Human)
    Tags
    x6 HIS and an IL2 secretion signal both N termina…
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Available Since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    226841-plasmid-map-sequence-id-456206

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  14. pMbd3EosHaloTAF_Hyg

    Plasmid
    #140958
    Purpose
    Targeting construct to make Mbd3-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    methyl-CpG binding protein 3 (Mbd3 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140958-plasmid-map-sequence-id-338424

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  16. pChd4EosHaloTAF_Puro

    Plasmid
    #172099
    Purpose
    Targeting construct to make Chd4-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    chromodomain helicase DNA binding protein 4 (Chd4 Mouse, Synthetic)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    172099-plasmid-map-sequence-id-338425

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  18. pSCM167-Flag-hSFRP1

    Plasmid
    #189538
    Purpose
    Protein expression of SFRP1 in mammalian cells.
    Depositor
    Haian Fu
    Insert
    secreted frizzled related protein 1 (SFRP1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Oct. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    189538-plasmid-map-sequence-id-374368

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  21. SP_H3

    Plasmid
    #228189
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P47001_CIS3_YEAST:0-21
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228189-plasmid-map-sequence-id-451647

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  22. SP_H4

    Plasmid
    #228190
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P80075_CCL8_HUMAN:0-23
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228190-plasmid-map-sequence-id-451651

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  23. SP_H5

    Plasmid
    #228191
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P31431_SDC4_HUMAN:0-20
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228191-plasmid-map-sequence-id-451636

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  24. SP_H6

    Plasmid
    #228192
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P59827_BPIB4_HUMAN:0-17
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228192-plasmid-map-sequence-id-451633

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  25. SP_F2

    Plasmid
    #228176
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast EXG1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P23776_EXG1_YEAST:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228176-plasmid-map-sequence-id-452512

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  26. SP_F3

    Plasmid
    #228177
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_B5VE42_ECM33_YEAS6:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228177-plasmid-map-sequence-id-451788

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  27. SP_F4

    Plasmid
    #228178
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human CCL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P13500_CCL2_HUMAN:0-23
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228178-plasmid-map-sequence-id-451792

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  28. SP_F5

    Plasmid
    #228179
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human MMP11 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P24347_MMP11_HUMAN:0-33
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228179-plasmid-map-sequence-id-452507

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  29. SP_F6

    Plasmid
    #228180
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human PDGFC gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q9NRA1_PDGFC_HUMAN:0-22
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228180-plasmid-map-sequence-id-451845

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  30. SP_G1

    Plasmid
    #228181
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P38844_DSE2_YEAST:0-21
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228181-plasmid-map-sequence-id-452508

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  31. SP_G2

    Plasmid
    #228182
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast PRY1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P47032_PRY1_YEAST:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228182-plasmid-map-sequence-id-452516

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  32. SP_G3

    Plasmid
    #228183
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast AWA1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q8TGE1_AWA1_YEASK:0-23
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228183-plasmid-map-sequence-id-451798

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  33. SP_G6

    Plasmid
    #228186
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human EPGN gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q6UW88_EPGN_HUMAN:0-20
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228186-plasmid-map-sequence-id-451783

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  34. SP_H1

    Plasmid
    #228187
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P32603_SPR1_YEAST:0-20
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228187-plasmid-map-sequence-id-476276

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  35. SP_H2

    Plasmid
    #228188
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast HPF1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q05164_HPF1_YEAST:0-23
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228188-plasmid-map-sequence-id-451650

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  36. SP_D1

    Plasmid
    #228163
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast MEL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P41945_MEL2_YEASX:0-18
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228163-plasmid-map-sequence-id-451791

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  37. SP_D2

    Plasmid
    #228164
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast DAN1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_P47178_DAN1_YEAST:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228164-plasmid-map-sequence-id-452515

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  38. SP_D3

    Plasmid
    #228165
    Purpose
    Plasmid contains the secretion signal peptide sequence of the yeast PST2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q12335_PST2_YEAST:0-21
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228165-plasmid-map-sequence-id-451738

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  39. SP_D4

    Plasmid
    #228166
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human LPAL2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q16609_LPAL2_HUMAN:0-21
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228166-plasmid-map-sequence-id-451634

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  40. SP_D5

    Plasmid
    #228167
    Purpose
    Plasmid contains the secretion signal peptide sequence of the human LUZP2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).
    Depositor
    Joseph Brock
    Article
    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86
    Insert
    sp_Q86TE4_LUZP2_HUMAN:0-19
    Use
    Synthetic Biology
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228167-plasmid-map-sequence-id-451781

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Showing: 1401 - 1440 of 5023 results