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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_031

Plasmid

#123970

Purpose

Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pME-mKate-P2A-APEX2-NS

Plasmid

#67670

Purpose

Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-NS

Use

Gateway multisite middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-TUBA1B_sgRNA

Plasmid

#183889

Purpose

pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBetaActin-KIF13aMotor-mScarlet-FKBP

Plasmid

#191337

Purpose

Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

KIF13aMotor-mScarlet-FKBP (KIF13A Human)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 1-639

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK7_013

Plasmid

#123880

Purpose

Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Use

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
MTK1_008

Plasmid

#123671

Purpose

Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector LS-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_030

Plasmid

#123969

Purpose

Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phiC31-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-DmrC

Plasmid

#61673

Purpose

Non-fluorescent, truncated kinesin-1 fused to DmrC linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-DmrC (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK1_009

Plasmid

#123672

Purpose

Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L1-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge