We narrowed to 1,270 results for: grna cloning vector

1. pFTK087

   Plasmid

   #171359

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TmiRc3_shURB5

   Plasmid

   #81066

   Purpose

   Gateway entry vector encoding shRNA to UBR5

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   shRNA targeting UBR5
   

   Use

   Cloning

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Blat-BSD

   Plasmid

   #163791

   Purpose

   The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TmiRc3_shNT

   Plasmid

   #81067

   Purpose

   Gateway entry vector encoding non-targeting shRNA

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   non-targeting shRNA
   

   Use

   Cloning

   Promoter

   N/A

   Available Since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-hU6-mU6-Cre2GO

   Plasmid

   #136907

   Purpose

   Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   BlasCyGO
   

   Use

   Lentiviral

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFTK093

   Plasmid

   #171365

   Purpose

   Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFTK086

   Plasmid

   #171358

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   P-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MS2-adRNA (RAB7A-GAC site)

   Plasmid

   #170140

   Purpose

   AAV vector carrying a MS2 adRNA targeting the RAB7A transcript

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-RAB7A(GAC)-MS2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   RPS5A

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pECNUS2

    Plasmid

    #184885

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pECNUS3

    Plasmid

    #184886

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECNUS1

    Plasmid

    #184884

    Purpose

    ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

    Depositor

    Eunyoung Chae

    Article

    Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJR107

    Plasmid

    #187245

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJR106

    Plasmid

    #187244

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJR103

    Plasmid

    #187242

    Purpose

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJR104

    Plasmid

    #187243

    Purpose

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
    

    Use

    Lentiviral

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p1071-Lenti-Single loxP

    Plasmid

    #217892

    Purpose

    Lentiviral vector, entry vector for Lentiviral Switch-OVER

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJR101

    Plasmid

    #187241

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJR100

    Plasmid

    #187240

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB3405

    Plasmid

    #106166

    Purpose

    EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge