We narrowed to 23,946 results for: nes

1. pNB776

   Plasmid

   #60117

   Purpose

   Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Use

   Unspecified

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNB754

   Plasmid

   #60157

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   LYS9pr

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNB691

   Plasmid

   #60135

   Purpose

   Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNB753

   Plasmid

   #60148

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   LEU1pr

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBUN82

   Plasmid

   #60675

   Purpose

   overexpression of M. smegmatis alrA gene in Mycobacteria

   Depositor

   Raul Barletta

   Article

   Caceres et al J Bacteriol. 1997 Aug;179(16):5046-55.

   Insert

   alrA
   

   Use

   E.coli-mycobacterium shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   hsp60

   Available since

   Dec. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pNB689

   Plasmid

   #60133

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNB756

   Plasmid

   #60149

   Purpose

   Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   YeLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   LEU1pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNB752

   Plasmid

   #60155

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   ADE17pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Use

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Integrin-β6 Headpiece I270C

    Plasmid

    #159638

    Purpose

    Contains integrin beta 6 residues 1–474 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

    Insert

    Integrin beta 6 headpiece I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCB6HindIII-rat FcRn

    Plasmid

    #121671

    Purpose

    Expresses rat neonatal Fc receptor in mammalian cells

    Depositor

    Pamela Bjorkman

    Article

    Tesar et al Traffic. 2006 Sep;7(9):1127-42.

    Insert

    rat neonatal Fc receptor (Fcgrt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSimpleII-NmCas9-FLAG

    Plasmid

    #47872

    Purpose

    FLAG tagged NmCas9 with no NLS.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQP-AR10

    Plasmid

    #138980

    Purpose

    Endogenous RAS regulation using light-controlled intrabody

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNB813

    Plasmid

    #60161

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Use

    Tags

    PEST degron

    Expression

    Yeast

    Mutation

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBUN279

    Plasmid

    #60674

    Purpose

    integration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB site

    Depositor

    Raul Barletta

    Article

    Feng et al J Bacteriol. 2002 Sep;184(18):5001-10.

    Insert

    ald
    

    Use

    E.coli-mycobacterium integration-proficient vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNB655

    Plasmid

    #60142

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V

    Promoter

    MET17pr

    Available since

    Feb. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNB695

    Plasmid

    #60144

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNB697

    Plasmid

    #60146

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge