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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 WT

Plasmid

#17690

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK9 (D167N) HA (P#811)

Plasmid

#14641

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant. Please see deposit…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AsnRS

Plasmid

#124105

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Asparaginyl-tRNA ligase (asnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-AspRS-His

Plasmid

#124106

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Aspartate-tRNA ligase (aspS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB454

Plasmid

#74836

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-ValRS-His

Plasmid

#124122

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Valyl-tRNA ligase (valS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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qs5

Plasmid

#24498

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hnRNPU-V5

Plasmid

#35974

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)

Tags

V5

Expression

Mammalian

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-RRN3/TIF1A

Plasmid

#17661

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RRN3/TIF1A (RRN3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

See depositor comments below

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Promoter

CaMKIIa

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pQC mPlum IX

Plasmid

#37355

Depositor

Connie Cepko

Article

Beier et al Dev Biol. 2011 May 15;353(2):309-20. Epu

Insert

mPlum

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-BRCA1

Plasmid

#115491

Purpose

EGFP-BRCA1 expression vector. Parton clone BXY

Depositor

Rob Parton

Article

McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

Insert

BRCA1 (BRCA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S1009R (See depositor comments)

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-EYFP

Plasmid

#104052

Purpose

An AAV genome encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.V1

Insert

EYFP

Use

AAV

Promoter

CAG

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP Rootletin (Nigg pFL2(CW499))

Plasmid

#41166

Depositor

Erich Nigg

Article

Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

Insert

Rootletin (CROCC Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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