Addgene

We narrowed to 1,468 results for: pBluescript

Showing: 701 - 720 of 1468 results

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pBS-NT-AscI

Plasmid

#15461

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

neurotensin (Nts Mouse)

Use

Tags

Expression

Mutation

Added AscI site 3 bp 3' of stop codon

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pDHJS3 AN-

Plasmid

#78241

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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14-3-3 KO vector

Plasmid

#16551

Depositor

Bert Vogelstein

Article

Chan et al Nature. 1999 Oct 7. 401(6753):616-20.

Insert

14-3-3 knockout (SFN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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3CB1/cSprouty2

Plasmid

#22096

Depositor

Gail Martin

Article

Minowada et al Development. 1999 Oct . 126(20):4465-75.

Insert

Sprouty 2 (SPRY2 Chicken)

Use

Bacterial

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pFloxin-Ofd1-Myc

Plasmid

#24558

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Expression

Mutation

includes exons 4-23 only

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
cpi-17

Plasmid

#78708

Purpose

Encodes zebrafish cpi-17

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

cpi-17 (ppp1r14aa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Use

Tags

Expression

Mutation

Promoter

cpc operon

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PL06010B1H03 (smedwi1)

Plasmid

#35699

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi1

Use

Tags

Expression

Mutation

Promoter

T7 sense, T3 antisense

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pBS RAI14

Plasmid

#19204

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Retinoic acid induced 14 (RAI14 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS Olfactomedin1

Plasmid

#19225

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Olfactomedin 1 (OLFM1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pBS WSB2

Plasmid

#19190

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

WSB2 (WSB2 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
fabp10a:DD-CreER; cryaa:mCerulean

Plasmid

#230076

Purpose

Expression of destabilized CreER in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Hovhannisyan et al bioRxiv 2025.05.23.655316

Insert

ltDHFR(DD)

Use

Zebrafish expression

Tags

CreER and HA

Expression

Mutation

amino acids mutations: R12H, N23S, G67S, V78A, E1…

Promoter

fabp10a

Available since

May 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry

Plasmid

#230062

Purpose

Expression of genetically encoded calcium chelator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

SpiCee

Use

Zebrafish expression

Tags

FLAG and mRFP1

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBSsgRNA

Plasmid

#224867

Purpose

Yeast genome editing, Insertion of 20 bp oligo into sgRNA gene

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro BFP nLAP +0

Plasmid

#194317

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(BFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag Hygro EGFP nLAP -2

Plasmid

#194322

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

Insert

Hygro-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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