-
Plasmid#105188PurposeModule 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)DepositorInsertG14p-mKate2-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1
Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2-BSD
Plasmid#191101PurposeLentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD markerDepositorInsertNRCH-PE2-BSD
UseCRISPR and LentiviralTagsSV40 NLSExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)
Plasmid#105201PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)DepositorInsertG8p-IL12-P2A-CCL21-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN472 (FuGW-G8p-STE-1Pe)
Plasmid#105190PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of STE transcripts containing miR1-Bs(Pe)DepositorInsertG8p-STE-1Pe
UseLentiviral and Synthetic BiologyTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains2
Plasmid#78460PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2264-2315; Q2513R (see deposi…PromoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains3
Plasmid#78459PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2497-2529PromoterT7Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLN539 (FuGW-G8p-antiPD1(HC)-P2A-antiPD1(LC)-1Pe)
Plasmid#105200PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-P2A-antiPD1(LC)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*
Plasmid#169850PurposeMammalian Expression, SpyCas9 prime editor, optimized NLSDepositorInsertspCas9 PE*
UseTagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianMutationPromotercmvAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC414 - pAAV EF1a hPREP
Plasmid#59967PurposeAn AAV packaging vector that expresses hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PTGS1-bio-His
Plasmid#53414PurposeExpresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTGS1 (PTGS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆GR-MET15
Plasmid#207033PurposeExpression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆GRPromoterpATG8Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)
Plasmid#105194PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(HC)-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)
Plasmid#105195PurposeModule 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)DepositorInsertG8p-antiPD1(LC)-1Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn
Plasmid#214102PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dnDepositorInsertPE-Plus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_T7-PE2-Nuclease
Plasmid#171997PurposeSequence provided for PE-Nuclease mRNA transcription, suitable for microinjectionDepositorInsertCMV_T7-Cas9-RT
UseTagsExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCMV for Cas9, U6 for gRNAsAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationS554APromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only