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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7181 - 7200 of 15617 results

  12. pAAV-hSyn-ChRger2-TS-EYFP

    Plasmid
    #127237
    Purpose
    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.
    Depositor
    Viviana Gradinaru
    Article
    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s
    Insert
    ChRger2-TS-EYFP
    Use
    AAV
    Tags
    EYFP and SpyTag
    Expression
    Mammalian
    Promoter
    hSyn1
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127237-plasmid-map-sequence-id-246998

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  14. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Available Since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

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  18. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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Showing: 7181 - 7200 of 15617 results