We narrowed to 26,296 results for: Vit

1. pPRISM-QF2-he1.1:YFP

   Plasmid

   #184819

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QF2-pA-he1.1:YFP
   

   Use

   CRISPR; Backbone for cloning of homology arms (fo…

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRISM-QF2-he1.1:YFP-rln3a-HA

   Plasmid

   #184820

   Purpose

   Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   rln3a-HA
   

   Use

   CRISPR; For integration of qf2 by homology-direct…

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGal4-DBD-OCT4_WT_N

   Plasmid

   #215622

   Purpose

   For overexpression of Gal4-DBD OCT4 WT N

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   OCT4 N
   

   Expression

   Mammalian

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p931-SWITCH-OVER insert: EF1a-Blasti

   Plasmid

   #217890

   Purpose

   insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p223-Pulse-Switch

   Plasmid

   #217889

   Purpose

   Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

   Plasmid

   #207171

   Purpose

   expression plasmid for Gal4DBD-HIF-CAD.myc expression

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   Gal4DBD-HIF1a-CAD Myc tag linker
   

   Tags

   2xMyc tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

   Plasmid

   #207172

   Purpose

   expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   Gal4DBD-HIF1a-CAD GalO linker
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lneg-INTR-GFP

   Plasmid

   #208902

   Purpose

   encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFP

   Depositor

   Christian Gruber

   Article

   Liutkeviciute et al FASEB J. 2018 Jun 25;32(12):fj201800443.

   Insert

   INTR
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGAL4-DBD-S6Y_AroPERFECT

   Plasmid

   #215631

   Purpose

   For overexpression of Gal4-DBD S6Y AroPERFECT

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   S6Y AroPERFECT
   

   Expression

   Mammalian

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGal4-DBD-OCT4_AroPERFECT_C

    Plasmid

    #215625

    Purpose

    For overexpression of Gal4-DBD OCT4 AroPERFECT C

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    OCT4 AroPERFECT C
    

    Expression

    Mammalian

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAL4-DBD-S6Y_AroPATCHY3

    Plasmid

    #215632

    Purpose

    For overexpression of Gal4-DBD S6Y AroPATCHY 3

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    S6Y AroPATCHY 3
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGal4-DBD-OCT4_WT_C

    Plasmid

    #215623

    Purpose

    For overexpression of Gal4-DBD OCT4 WT C

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    OCT4 C
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAL4-DBD-S6Y_AroPATCHY1

    Plasmid

    #215633

    Purpose

    For overexpression of Gal4-DBD S6Y AroPATCHY1

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    S6Y AroPATCHY1
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGal4-DBD-OCT4_AroPERFECT_N

    Plasmid

    #215624

    Purpose

    For overexpression of Gal4-DBD OCT4 AroPERFECT N

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    OCT4 AroPERFECT N
    

    Expression

    Mammalian

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC-T7-hTP53-P0~670-FLuc

    Plasmid

    #208640

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

    Depositor

    Quanfu Ma

    Insert

    TP53, UTR and partial CDS (TP53 Human)
    

    Use

    Luciferase; In vitor transcription template

    Tags

    Firefly Luciferase (F-Luc) and T7 promoter

    Mutation

    UTR and partial CDS in-frame with F-Luc

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. hPRL2_1-163

    Plasmid

    #209794

    Purpose

    Bacterial expression of human PRL-2 without farnesylation motif

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

    Insert

    PRL-2 (PTP4A2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    aa 1-163 only

    Promoter

    T7

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMx/ATP8B4-HA

    Plasmid

    #209229

    Purpose

    Mammalian expression of ATP8B4

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B4 (ATP8B4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMx/ATP8B3-HA

    Plasmid

    #209228

    Purpose

    Mammalian expression of ATP8B3

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP8B3 (ATP8B3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. RS02060_pMQ30_KO_Kan

    Plasmid

    #185391

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS02060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS02060 upstream flanking homology region
    RR42_RS02060 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. RS28935_pMQ30_KO_Kan

    Plasmid

    #185392

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS28935 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS28935 upstream flanking homology region
    RR42_RS28935 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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