We narrowed to 18,160 results for: Eomes

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phNFL-E396K

Plasmid

#132595

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

1186G>A; Glu396Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTS1026-Tier1-PhCMV-3xNLS-iRFP670

Plasmid

#169532

Purpose

Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized far-red fluorescent protein iRFP670

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-Gamma 5

Plasmid

#36044

Depositor

Narasimhan Gautam

Article

Akgoz et al Cell Signal. 2006 Oct;18(10):1758-68. Ep

Insert

G protein subunit Gamma 5 (Gng5 Rat)

Tags

His and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pCIneo-PAM-sig-His6-gamma-PALcc-deltaGlyc

Plasmid

#25458

Depositor

Betty Eipper

Article

Chufan et al Structure. 2009 Jul 15. 17(7):965-73.

Insert

PAM (Pam Rat)

Tags

His6 and PAM signal

Expression

Mammalian

Mutation

Contains aa 498-820 (PALcc= PAL catalytic core) …

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
LP380

Plasmid

#90206

Purpose

pEGFP-C1 with cDNA encoding HsDLEC1 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

DLEC1 (DLEC1 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB416

Plasmid

#68681

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CMV10-Flag-mMcl-1 KR

Plasmid

#32979

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Tags

3XFlag

Expression

Mammalian

Mutation

All lys changed to Arg

Promoter

CMV

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only

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pUHG10.3-TetO-Rs1

Plasmid

#16313

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Changed Aspartic Acid 100 to Alan…

Available Since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-N-18

Plasmid

#57690

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

NM_003380.3 (VIM Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcFLAG-Grap2-WT

Plasmid

#74511

Purpose

Overexpression of Grap2 in mammalian cells

Depositor

Lars Rönnstrand

Article

Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

Insert

Grap2 (GRAP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKsubDD-YPet-NES

Plasmid

#84633

Purpose

Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

JNKsubDD-YPet-NES

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD1-deletion

Plasmid

#75265

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD1 (activation domain 1) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-AD2-deletion

Plasmid

#75266

Purpose

Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

TTF-1 (NKX2-1 Human)

Tags

none

Expression

Mammalian

Mutation

AD2 (activation domain 2) deleted

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJAF216

Plasmid

#52405

Purpose

Expresses Flag-tagged mouse Arf4

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf4 (Arf4 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-β-tubulin-Sirius

Plasmid

#175825

Purpose

Express Sirius with microtubule-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with microtuble-targeting sequence

Tags

human tubulin beta 4B class IVb;TUBB4B

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-E2Crimson

Plasmid

#153425

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-SECFP

Plasmid

#174470

Purpose

Express SECFP with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with golgi apparatus-targeting sequence

Tags

β1,4-galactose transferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-Tom20-SECFP

Plasmid

#174185

Purpose

Express SECFP with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with mitchondria-targeting sequence

Tags

the mitochondria-targeting sequence of TOM20

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-249

Plasmid

#53549

Purpose

mammalian expression of TPP1 OB-fold domain

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa 88-249

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3_HLA-cmyc-GRM3 C127A

Plasmid

#140451

Purpose

Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

Depositor

Erik Procko

Insert

GRM3 (GRM3 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

Cysteine 127 to alanine

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/SPTBN1-FLT3

Plasmid

#168115

Purpose

Mammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemia

Depositor

Nicholas Cross

Article

Grand et al Exp Hematol. 2007 Nov;35(11):1723-7. doi

Insert

SPTBN1-FLT3

Expression

Mammalian

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKAsub-YPet-NES

Plasmid

#138203

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKAsub-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pVenus_Lys-less-Cdc20 (1620)

Plasmid

#39849

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Tags

Venus

Expression

Mammalian

Mutation

Lys-less = all Lys converted to Arg

Available Since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-153

Plasmid

#26330

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-153 (Mir153 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCALNL-eGFP-NT1-6-gix-6-NT1

Plasmid

#81204

Purpose

contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

gix-Neo-gix deletion cassette upstream of EGFP

Expression

Mammalian

Promoter

pCBa

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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