We narrowed to 63,682 results for: cell

1. FASTHDR-Cterm-NanoLuc-Puromycin

   Plasmid

   #167209

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NanoLuc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-MCS-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80058

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. BTK-APEX2-P2A-mKate2

   Plasmid

   #67653

   Purpose

   Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   BTK-PH-APEX2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78306

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 Fyn SH2:R176E

   Plasmid

   #16035

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SH2 point mutant: R176E

   Promoter

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pX458-sgRNA_Ago1_1

   Plasmid

   #73533

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458-sgRNA_Ago1_2

   Plasmid

   #73534

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA1-Bystin

   Plasmid

   #11869

   Depositor

   Michiko Fukuda

   Article

   Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

   Insert

   Bystin (BYSL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA-Cx43-Myc

   Plasmid

   #60693

   Purpose

   mammalian expression of Myc-tagged rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEN_TT JunD-HA

    Plasmid

    #58498

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLKO.1-sh-mSox10-2

    Plasmid

    #37007

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSox10-2 (Sox10 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-TPD52

    Plasmid

    #172452

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal FLAG tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIneoLuc-PP1A

    Plasmid

    #128348

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Kawano et al J Biochem. 2015 May 14. pii: mvv051.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. MO70-hCRACR2A-isoform-c

    Plasmid

    #79591

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

    Insert

    CRACR2A-isoform-c (CRACR2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pENTR-COIL

    Plasmid

    #16172

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Coilin (COIL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

17. TRE-UBC-Crry-T2A-GFP

    Plasmid

    #205452

    Purpose

    lentiviral plasmid for expression of mouse Crry

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Crry (Cr1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hiv7CMV-Cremyc-2A-GFP

    Plasmid

    #117148

    Purpose

    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

    Depositor

    Qiang Lu

    Article

    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

    Insert

    Cremyc-2A-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSG5-HA-H-Ras_wt

    Plasmid

    #39509

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Eukaryotic

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pX458-sgRNA_Ago2_3

    Plasmid

    #73531

    Purpose

    Expresses SpCas9, GFP, and sgRNA targeting Ago2

    Depositor

    Constance Ciaudo

    Article

    Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

    Insert

    AGO2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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