We narrowed to 18,247 results for: multi
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Plasmid#224489PurposeGateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSun1-2xsfGFP-6xMYC-pA
UseGateway CloningAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFmcs-2H12.D11-scarREF
Plasmid#221184PurposecdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)DepositorInsertscdGreen2
mScarlet-I
ExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterNone (same as cdGreen2) and PQ5 (cumate-inducible)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM1-DuET
Plasmid#213207PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHRM1 (CHRM1 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CHRM2-DuET
Plasmid#213208PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHRM2 (CHRM2 Human)
Tags1D4 and FLAGExpressionMammalianPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-switch
Plasmid#195557PurposeTemperature state switch expressing low-temperature mWasabi and high-temperature mRFP1DepositorInsertsmRFP1
mWasabi
TcI39
Tags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHLP712
Plasmid#203741PurposeIntegration of OsTIR1(F74A)-3xMyc at NEUT5L locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOsTIR1
UseCandida albicans integrating vectorTags3xMycMutationCodon 74 changed from Phe to Ala (F74A)PromoterACT1 (C. albicans)Available sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-ECFP-Actb
Plasmid#169787PurposeAn actin FRET sensor. Must be expressed with Venus-actin for FRET.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertActb (Actb Mouse)
TagsECFPExpressionMammalianAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE_ProB(I69E)A
Plasmid#117756PurposeProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backboneDepositorInsertGlutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
ExpressionBacterialPromoterPLac(UV5)Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTurquoise2-WPRE
Plasmid#104103PurposeExpresses mTurquoise2 under the TRE promoter in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTurquoise2
ExpressionMammalianPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-TRE-EYFP-WPRE
Plasmid#104104PurposeExpresses EYFP under the TRE promoter in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEYFP
ExpressionMammalianPromoterTREAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pROS15
Plasmid#107929Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMEL12
Plasmid#107918Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOEM-GED-EGFP
Plasmid#102782PurposeBaculovirus rescue vector, VSVGED pseudotype, eGFPDepositorInsertEGFP
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianPromoterCMVAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgAtm/Cre
Plasmid#89643PurposeExpresses an Atm-targeting gRNA and Cre-recombinaseDepositorAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CeNL-Clathrin_pEGFP
Plasmid#89540PurposeCyan color variant of bright luminescent protein, fused to clathrin light chainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCeNL
TagsClathrin light chainExpressionMammalianPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-GAL1
Plasmid#85778PurposeN-ICE plasmid pK3FS-GAL1DepositorInsertloxP-KanMX-loxP-GAL1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CERT-PH
Plasmid#49572Purposeexpresses the plekstrin homology domain of CERT/OSBP in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPBP (CERT1 Human)
TagsEGFPExpressionMammalianMutationexpresses AA 1-122PromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR1.1
Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
TagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only