165,313 results

1. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Expression

   Yeast

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

   Plasmid

   #109995

   Purpose

   Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti6.3/TO/CD9-GFP

   Plasmid

   #104402

   Purpose

   Expresses CD9 with mEmerald tag (NOT GFP) at the N terminal

   Depositor

   Jens Gruber

   Article

   Boker et al Mol Ther. 2018 Feb 7;26(2):634-647. doi:

   Insert

   CD9 (CD9 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   CMV

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCGN-ELK-1

   Plasmid

   #27156

   Purpose

   For mammalian expression of human Elk-1 with an HA tag

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. PT2/C-Luc//PGK-SB13

   Plasmid

   #20207

   Depositor

   John Ohlfest

   Article

   Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

   Insert

   Flirefly luciferase/Sb transposase
   

   Use

   Transposon

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-THT

   Plasmid

   #55790

   Purpose

   GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Type

   Empty backbone

   Use

   RNAi; Gateway

   Expression

   Mammalian

   Promoter

   modified H1 RNA gene

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. bactin2:loxP-STOP-loxP-DsRed-express

   Plasmid

   #68797

   Purpose

   For making transgenic fish

   Depositor

   David Traver

   Article

   Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

   Inserts

   beta-actin promoter
   loxp-stop-codon-loxp-dsred
   

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-IQGAP1

   Plasmid

   #30112

   Depositor

   David Sacks

   Article

   Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

   Insert

   IQGAP1 (IQGAP1 Human)
   

   Tags

   tandem EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Vimentin-7

   Plasmid

   #55156

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

   Insert

   Vimentin (VIM Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. TFORF0346

    Plasmid

    #143397

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ChlorON-1

    Plasmid

    #193477

    Purpose

    Turn-on fluorescent sensor for chloride

    Depositor

    Sheel Dodani

    Article

    Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

    Insert

    ChlorON-1
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Flag-Tet1 CD

    Plasmid

    #70129

    Purpose

    Express flag-tagged mouse Tet1 catalytic domain.

    Depositor

    Yi Zhang

    Article

    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

    Insert

    Ten-Eleven Translocation 1 (Tet1 Mouse)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    The plasmid expresses TET1 catalytic domain.

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL-MND-hCard11 WT-T2A-GFP

    Plasmid

    #163787

    Purpose

    Expression of Wild-type CARD11

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Promoter

    MND

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTHSSe_45

    Plasmid

    #109243

    Purpose

    pSC101 var3 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_22A

    Plasmid

    #91133

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTagRFP657-N1

    Plasmid

    #31959

    Depositor

    Vladislav Verkhusha

    Article

    Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

    Insert

    TagRFP657
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. EYFP Fibronectin

    Plasmid

    #56600

    Purpose

    Localization: Extracellular Matrix, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    Fibronectin
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fibronectin begins amino acids at amino acid 900 …

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid

    #115684

    Purpose

    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

    Depositor

    David Suter

    Article

    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

    Insert

    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    

    Use

    Lentiviral

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA Flag Lamp2b-HA

    Plasmid

    #71292

    Purpose

    Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

    Depositor

    Joshua Leonard

    Article

    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

    Insert

    Lamp2b (LAMP2 Human)
    

    Tags

    Flag, HA, and Lamp2 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. Flag-neo-PP1-Gamma

    Plasmid

    #171270

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1

    Depositor

    Steven Balk

    Insert

    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLIB-WDR61

    Plasmid

    #231729

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Insert

    WDR61 (SKIC8 Human)
    

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAd5-B6/7 ∆E3-GFP

    Plasmid

    #179202

    Purpose

    A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.

    Depositor

    Fred Bunz

    Article

    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

    Insert

    Modified block 6/7, with deletion in E3 and insertion of GFP cassette
    

    Use

    Adenoviral

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGG-BC-TurboID-GOI

    Plasmid

    #209390

    Purpose

    Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pGG-GOI-DE-TurboID

    Plasmid

    #209388

    Purpose

    Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLentiCrispr-V2-mOrange (V2mO)

    Plasmid

    #140206

    Purpose

    Lenti Crispr/Cas9 with mOrange fluorescent marker

    Depositor

    Jaffer Ajani

    Article

    Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    pELF1a

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. L1-neo-TET

    Plasmid

    #51284

    Purpose

    expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    L1RE1 (L1RE1 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pEGFP-MRLC1

    Plasmid

    #35680

    Depositor

    Tom Egelhoff

    Article

    Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

    Insert

    MRLC1 (MYL9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pCMV-Flag-YAP-5SA/S94A

    Plasmid

    #33103

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

    Insert

    YAP (YAP1 )
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S61A, S94A, S109A, S127A, S164A, S381A

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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