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YES1 (T348I) in pLX303

Plasmid

#42564

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T348I

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF4-2xCSTREP

Plasmid

#136169

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF4

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF4

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAnc80L65

Plasmid

#68837

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct. Note that an improved version is available: Addgene plasmid# 92307. See comments for details

Depositor

Luk Vandenberghe

Article

Zinn et al Cell Rep. 2015 Jul 28. pii: S2211-1247(1

Insert

Ancestral AAV Capsid Anc80L65

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe zeo HMGA2

Plasmid

#17411

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

HMGA2 (Hmga2 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE

Plasmid

#130995

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-mScarlet under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

mScarlet-Kv2.1

Promoter

hSyn

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSMT3_SRSF1

Plasmid

#201056

Purpose

Express sumo-tagged human SRSF1

Depositor

Jun Zhang

Article

Fargason et al Elife. 2023 Mar 2;12:e84412. doi: 10.755

Insert

SRSF1 (SRSF1 Human)

Tags

sumo

Expression

Bacterial

Mutation

none

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Article

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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INPP5D-eGFP WT

Plasmid

#161998

Purpose

Mammalian expression vector, containing a GFP tagged version of INPP5D

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP5D (INPP5D Human)

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Luciferase-CD4-bla

Plasmid

#189981

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mCsnk2b - 1

Plasmid

#198505

Purpose

lentiviral stable expression of mCsnk2b gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCsnk2b (Csnk2b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-hCGN

Plasmid

#236507

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

Plasmid

#226577

Purpose

Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

I178A, L182A, L186A, V190A

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB2F

Plasmid

#195289

Purpose

Bicistronic HSV-TK and mScarlet resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

HSV-TK 2A mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Irf4

Plasmid

#139804

Purpose

doxycycline-inducible expression of mouse Irf4 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf4 (Irf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mScarlet-TUBA1B

Plasmid

#207764

Purpose

Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mLama1 AAV sgRNA 1, 2, 5

Plasmid

#135339

Purpose

Expression plasmid of VP64-SadCas9 sgRNA 1, 2 and 3

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

Lama1 VP64-dCas9 sgRNA Guides

Use

AAV

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF39-2xCSTREP

Plasmid

#136200

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF39

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-TUBA1B

Plasmid

#227327

Purpose

Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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