We narrowed to 22,696 results for: Sis;

1. NELFE-mOrange pHis-//

   Plasmid

   #53303

   Purpose

   Expresses His-tagged NELF-E-mOrange fusion protein.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   NELF-E protein (Nelf-E Fly)
   

   Use

   Tags

   His and mOrange

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCaSpeR4 mito roGFP2-Orp1

   Plasmid

   #65001

   Purpose

   Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB419

   Plasmid

   #74813

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPRv1.mir451_KO

   Plasmid

   #164689

   Purpose

   For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   miR-451 CRISPR KO gRNA (MIR451A )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL3.7 shRNA against CRTC2

   Plasmid

   #72711

   Purpose

   Lentiviral expression of shRNA against CRTC2 (3rd generation)

   Depositor

   Freddy Jeanneteau

   Article

   Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   CRTC2
   

   Use

   Lentiviral

   Tags

   CMV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-HA-hTRAF3 333-C

   Plasmid

   #44035

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 334-568; S393G and K492N

   Promoter

   Available Since

   April 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pHH0103_MYO15A-1/2

   Plasmid

   #91491

   Purpose

   Protein expression and purification of human SH3 domain construct MYO15A-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   MYO15A-1/2 (MYO15A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-mCherry2-G4

   Plasmid

   #164167

   Purpose

   Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

   Depositor

   Isei Tanida

   Article

   Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

   Insert

   mCherry2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB522

   Plasmid

   #74864

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-FDXR wt

    Plasmid

    #16610

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    FDXR (FDXR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. MDH1-PGK-GFP-miR9

    Plasmid

    #25036

    Depositor

    Bob Weinberg

    Article

    Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

    Insert

    mir-9-3 (MIR9-3 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 7, 2010

    Availability

    Academic Institutions and Nonprofits only

12. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2mFoxd3

    Plasmid

    #37267

    Depositor

    Patricia Labosky

    Article

    Steiner et al Development. 2006 Dec;133(24):4827-38. E

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Overexpression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pJMP1161

    Plasmid

    #119251

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLexm-gfp-Pept2

    Plasmid

    #167988

    Purpose

    expresses rat PepT2 with a c terminal cleavable GFP-His tag.

    Depositor

    Simon Newstead

    Article

    Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

    Insert

    PepT2 (Slc15a2 Rat)
    

    Use

    Tags

    cleavable GFP-his8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-hSyn-IntCCCre-T2A-CopGFP

    Plasmid

    #160503

    Purpose

    Expresses IntCCCre in neurons in split intein-mediated split-Cre recombinase applications

    Depositor

    Shawn Je

    Article

    Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

    Insert

    IntCCCre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    CopGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    March 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

19. p6903 PHAGE-B CMV-N-EGFP Myc

    Plasmid

    #37608

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    Myc (MYC Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pX458_miR-290-295KO-gRNA1

    Plasmid

    #172709

    Purpose

    Cas9 with 5' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pX458_miR-290-295KO-gRNA2

    Plasmid

    #172710

    Purpose

    Cas9 with 3' gRNA for paired CRISPR KO of miR-290-295 cluster

    Depositor

    Constance Ciaudo

    Article

    Schaefer et al EMBO Rep. 2022 Jul 28:e54762. doi: 10.15

    Insert

    miR-290-295
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

    Plasmid

    #127360

    Purpose

    gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJMP1071

    Plasmid

    #119245

    Purpose

    rfp "test" strain

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_VAV2-2/2

    Plasmid

    #91506

    Purpose

    Protein expression and purification of human SH3 domain construct VAV2-2/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV2-2/2 (VAV2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p156 pWZL-EGFP-SLPI

    Plasmid

    #11069

    Depositor

    Ramesh Shivdasani

    Article

    Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

    Insert

    SLPI (Slpi Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pT2-cryR;tmem88aCE1-P1Egfp

    Plasmid

    #90148

    Purpose

    contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    tmem88aE1P1Egfp (tmem88a Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET23b-Casp7 C186A-His

    Plasmid

    #11826

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 7 catalytic mutant (CASP7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C186A

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

28. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

    Plasmid

    #192676

    Purpose

    Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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