We narrowed to 12,086 results for: HAL;

1. pOPINK-OTUD1 (OTU, aa 287-437)

   Plasmid

   #61407

   Purpose

   Expresses human OTUD1 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286 and aa 438-481.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMuLE ENTR CMV CreERT2 R4-R3

   Plasmid

   #62173

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and CreERT2 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   CreERT2
   

   Use

   Mule gateway entry vector

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PhyB-mCherry-Spc72

   Plasmid

   #51582

   Purpose

   Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   Spc72 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-mRFP1 gRNA

   Plasmid

   #117052

   Purpose

   In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   anti-mRFP1 SpCas9 gRNA
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL0373 (His-MBP-TniQ)

   Plasmid

   #130638

   Purpose

   Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ
   

   Use

   CRISPR; Transposon

   Tags

   His10-MBP-TEVsite on TniQ

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. BTK-APEX2-P2A-mKate2

   Plasmid

   #67653

   Purpose

   Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   BTK-PH-APEX2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRuby2-H2B-6

   Plasmid

   #55897

   Purpose

   Localization: Nucleus/Histones, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   H2B (H2BC11 Human)
   

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pFUW-TetO-SUB1

   Plasmid

   #178440

   Purpose

   doxycycline-inducible expression of Human SUB1 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   SUB1 (SUB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-β-arrestin2 RRK/Q

   Plasmid

   #38261

   Depositor

   Robert Lefkowitz

   Article

   Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

   Insert

   β-arrestin2 (Arrb2 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Arg233, Arg237, and Lys251 converted to Glutamines

   Promoter

   CMV

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

10. CoV2-N-M234I

    Plasmid

    #177955

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (M234I) (N )
    

    Expression

    Mammalian

    Mutation

    M234I

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-PAZ(aa1-480)

    Plasmid

    #21529

    Depositor

    Edward Chan

    Article

    Lian et al RNA. 2009 May . 15(5):804-13.

    Insert

    Ago2 (AGO2 Human)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    contain only aa1-480

    Available Since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pOPINK-OTUB2 (full-length, aa 1-234)

    Plasmid

    #61421

    Purpose

    Expresses human OTUB2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUB2 (Ubiquitin thioesterase OTUB2) (OTUB2 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIBN-EYFP

    Plasmid

    #103815

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. DJ-1 TEV site pET15b

    Plasmid

    #60687

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    DJ-1 (PARK7 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    TEV site was inserted (see comments below).

    Promoter

    T7

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pBI101-pGC1(D1)-GUS

    Plasmid

    #49513

    Purpose

    Expresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plant

    Depositor

    Julian Schroeder

    Article

    Yang et al Plant Methods. 2008 Feb 19;4:6. doi: 10.

    Insert

    Promoter of GC1(D1) (AT1G22690 Mustard Weed)
    

    Use

    Plant expression

    Tags

    beta-glucuronidase

    Promoter

    GC1 (At1g22690), 1141 bp upstream of the transcri…

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. FLAG-FLJ20309

    Plasmid

    #15360

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    FLJ20309 106-544residues (INO80D Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-iCre-2A-venus

    Plasmid

    #182499

    Purpose

    Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    iCre-2A-Venus
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    fragment of histidine decarboxylase gene promoter…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEST-p70a-UTR3-deGFP-6xHis-T500

    Plasmid

    #92224

    Purpose

    Expression of deGFP using the p70a promoter and UTR3

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    deGFP
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE ENTR CMV LacZ L5-L2

    Plasmid

    #62166

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and LacZ module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    LacZ
    

    Use

    Mule gateway entry vector

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIBN-mCherry

    Plasmid

    #103816

    Purpose

    Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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