177,602 results
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Plasmid#110847PurposeLentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterTRE3GAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LLP339 pGK-dCas9-SunTag
Plasmid#100943PurposedCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1aDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHAExpressionMammalianMutationD10A, H840A for dCas9Available sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-eGFP-mcGAS
Plasmid#108675PurposeFor production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGASDepositorAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CXCL12-sfGFP
Plasmid#98961PurposeMammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCXCL12 (CXCL12 Human)
TagsSuperfolder GFPExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQUAST-attB
Plasmid#104880PurposeFor insertion of 5xQUAS-geneX transgenes using PhiC31 integrase systemDepositorTypeEmpty backboneExpressionInsectAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.hSynapsin.SF-iGluSnFR.A184S
Plasmid#106174PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas serviceAAV1 and AAV9InsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterhSynapsinAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CBh-mKate2-IRES-MCS (WT-WT)
Plasmid#105921PurposeAAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRsDepositorHas serviceAAV2InsertsmKate2
IRES-Multiple cloning site
UseAAVMutationcodon optimizedPromoterCBh and same CBh promoter as mKate2Available sinceFeb. 15, 2018AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
phage UbiC NLS HA stdMCP stdHalo
Plasmid#104999PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag for mRNA labelingDepositorInsertstdMCP-stdHalo
UseLentiviralTagsHA tag, NLS, Two synonymous copies of the Halo-ta…ExpressionBacterial and MammalianPromoterUbiCAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N-Drd1
Plasmid#104358PurposeExpresses mouse dopamine receptor subtype 1 with EGFP fused to the C-terminus of the receptor. Localizes to primary cilia.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDopamine receptor D1 (Drd1) (Drd1 Mouse)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64
Plasmid#85424PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-7 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-7_synNotch
UseLentiviralPromoterPGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCW57.1-Luciferase
Plasmid#99283PurposeAll-in-one lentivector with tet-inducible luciferaseDepositorInsertFirefly luciferase
UseLentiviralExpressionMammalianPromoterTREAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMGF182
Plasmid#97006PurposeExpresses GFP-CHMP7 in mammalian cells under a crippled promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCHMP7 (CHMP7 Human)
TagsGFPExpressionMammalianPromoterCMV delta 5Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hAsCpf1(TYCV) (pY210)
Plasmid#89351PurposeExpresses humanized AsCpf1 variant that recognizes TYCV PAMsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCpf1, RR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K607RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-VP6SA11
Plasmid#89166PurposeExpress Rotavirus SA11 VP6 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 VP6
ExpressionMammalianPromoterT7 promotorAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2]
Plasmid#84446PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.DepositorHas serviceAAV1, AAV5, and AAV8InsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCAGAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-NSP4SA11
Plasmid#89171PurposeExpress Rotavirus SA11 NSP4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRotavirus SA11 NSP4
ExpressionMammalianPromoterT7 promotorAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GRB2-GFP
Plasmid#86873PurposeTo express GRB2 with a GFP tag at C terminus.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGRB2 (GRB2 Human)
TagsGFPExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2 mCherry-Rtn4a
Plasmid#86683PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorAvailable sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-Vps34
Plasmid#86749PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Vps34 (PIK3C3 Human)
TagsHAExpressionMammalianPromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by