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MSCV-Puro-FH-AGO2-S828A

Plasmid

#91991

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E

Plasmid

#91994

Purpose

Expresses FLAG-HA-AGO2 (S824E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S828E

Plasmid

#91995

Purpose

Expresses FLAG-HA-AGO2 (S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Human NCKX3 (pcDNA3.1+)

Plasmid

#75205

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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POT2-CYC1p-ycrtE-ADH1t

Plasmid

#65354

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtE-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Hoxa4-3'UTR

Plasmid

#31524

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxa4-3'UTR (Hoxa4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196a-1SM-PGK-GFP-Blast

Plasmid

#31510

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196a1 (Mir196a-1 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196a-2-PGK-GFP-Blast

Plasmid

#31511

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196a2 (Mir196a-2 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-324SM-PGK-GFP-Blast

Plasmid

#31518

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-324 (Mir324 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-324-PGK-GFP-Blas

Plasmid

#31517

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-324 (Mir324 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196bSM-PGK-GFP-Blast

Plasmid

#31514

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196b (Mir196b Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
MDH1-miR-196b-PGK-GFP-Blast

Plasmid

#31513

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-196b (Mir196b Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pLV-hCCND1-T286A-HA

Plasmid

#174155

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Mutation

A>G at base 856 (Thr>Ala at amino acid 286)

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-deltaH-AMBRA1-3xFLAG

Plasmid

#174158

Purpose

Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Missing aa 1-34

Promoter

CMV

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_PGE2mut-IRES-mCherry-CAAX

Plasmid

#251354

Purpose

Express the PGE2-insensitive control sensor GRAB_PGE2mut in mammalian cells

Depositor

Yulong Li

Article

Wang et al bioRxiv [Preprint]. 2025 Jun 3:2025.06.0

Insert

PGE2-insensitive sensor GRAB_PGE2mut (control sensor)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_PGE2-1.0-IRES-mCherry-CAAX

Plasmid

#251353

Purpose

Express the PGE2 sensor GRAB_PGE2-1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al bioRxiv [Preprint]. 2025 Jun 3:2025.06.0

Insert

PGE2 sensor GRAB_PGE2-1.0

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pPtGE31_ΔPtR

Plasmid

#236260

Purpose

A version of the episome pPtGE31 with a deleted nonessential region, contains the NAT cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pPtGE31_ShBle

Plasmid

#236261

Purpose

A version of the episome pPtGE31_ΔPtR with an added ShBle cassette

Depositor

Bogumil Karas

Article

Walker et al Nat Commun. 2026 Jan 22. doi: 10.1038/s4

Type

Empty backbone

Use

Synthetic Biology; P. tricornutum expression

Expression

Bacterial and Yeast

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge