We narrowed to 7,669 results for: CCH

1. pcDNA3. Flag-CSL R192H

   Plasmid

   #167005

   Purpose

   Mammalian expression of CSL R192H

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R192H

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYX233-b2-DHFR

   Plasmid

   #163759

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2-DHFR (Dhfr Budding Yeast, Mouse)
   

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVtrunc sfCherry2ΔC4-Lifeact

   Plasmid

   #231557

   Purpose

   Mammalian expression of Lifeact fused to C-terminally truncated superfolder Cherry2, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   Lifeact (ABP140 Budding Yeast)
   

   Tags

   sfCherry2

   Expression

   Mammalian

   Promoter

   CMVtrunc

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

   Plasmid

   #42651

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 399 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T399I

   Promoter

   CMV/TO

   Available Since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

   Plasmid

   #99049

   Purpose

   Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

   Depositor

   Barak Cohen

   Article

   Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

   Inserts

   ACT1 promoter (ACT1 Budding Yeast)
   mCherry
   Zif268 DNA binding Domain
   Estrogen response domain (ESR1 Human)
   

   Expression

   Yeast

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T2-Ste7-EE

   Plasmid

   #52681

   Purpose

   expression of constitutively active Ste7 kinase in bacteria

   Depositor

   Benjamin Turk

   Article

   Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

   Insert

   Ste7 (STE7 Budding Yeast)
   

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   S359E/T363E constitutively active

   Promoter

   tac

   Available Since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

7. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

   Plasmid

   #42650

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 299 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D299G

   Promoter

   CMV/TO

   Available Since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pvalb-2A-Flpo Flp-in replacement vector

   Plasmid

   #61572

   Purpose

   Recombinase-mediated cassette exchange in ES cells to insert the Flpo recombinase gene at the mouse Pvalb gene stop codon

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Pvalb-2A-Flpo
   

   Use

   Recombinase-mediated cassette exchange using flp …

   Available Since

   Feb. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-Gal4-dCas9-VP64

   Plasmid

   #71128

   Purpose

   This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.

   Depositor

   Ronald Davis

   Article

   Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.

   Insert

   Gal4-dCas9-VP64
   

   Tags

   NLS n-terminal, N terminal Gal4 Activator domain,…

   Expression

   Yeast

   Promoter

   Tef1

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLINC-AKAR1(TA)-CAAX

    Plasmid

    #87706

    Purpose

    Negative-control mutant of FLINC-AKAR1-CAAX.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FLINC-AKAR1(TA)-CAAX
    

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid

    #159088

    Purpose

    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Inserts

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    

    Use

    Fluorescent reporter

    Promoter

    1.7kb fabp6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX307_PDHA2S293A

    Plasmid

    #115197

    Purpose

    Lentiviral transduction and expression of PDHA2S293A into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    PDHA2S293A (PDHA2 Human)
    

    Use

    Lentiviral

    Mutation

    p.S293A

    Promoter

    EF1alpha

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPHAGE-EF1α-Luc-C20orf24-3’UTR-WT

    Plasmid

    #128551

    Purpose

    Lentiviral constitutive expression of Firefly luciferase under control of WT 3'UTR of human C20orf24.

    Depositor

    Mohan Babu

    Article

    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTO_Snf7_LAP

    Plasmid

    #101851

    Purpose

    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    SNF7 (SNF7 Budding Yeast)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV phSyn1(S)-FlpE-bGHpA

    Plasmid

    #50364

    Purpose

    Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    FlpE recombinase gene
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. shCCL20

    Plasmid

    #65096

    Purpose

    retroviral expression of CCL20 shRNA

    Depositor

    Pau Sancho-Bru

    Article

    Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

    Insert

    Ccl20 shRNA (Ccl20 Mouse)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. hu-TLR4 299 snp-flag pDEST40

    Plasmid

    #42647

    Purpose

    destination vector for expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. macroH2A2-GFP (pc2191)

    Plasmid

    #223709

    Purpose

    Expression of macroH2A2 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. macroH2A1.2-F192V-GFP (pc5115)

    Plasmid

    #223438

    Purpose

    Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A1.2-F192V (Macroh2a1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of phenylalanine 192 to valine (F192V)

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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