We narrowed to 15,732 results for: LEA

1. YFP NLS Beta-Actin S14C

   Plasmid

   #60614

   Purpose

   Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   S14C

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBridge-tyrosinase.σ2

   Plasmid

   #197415

   Purpose

   Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Inserts

   tyrosinase cytosolic tail
   AP-2 σ2
   

   Tags

   GAL4-DNA binding domain fragment, HA tag, and nuc…

   Expression

   Yeast

   Mutation

   encodes R42G substitution, contains silent substi…

   Promoter

   ADH1 and MET25

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-RasAR(R89L)-NES

   Plasmid

   #205568

   Purpose

   RasAR negative-control mutant plasmid. Targeted to cytosol.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   RasAR(R89L)-NES (RAF1 Synthetic, Human)
   

   Tags

   Nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Arg 89 mutated to Leu in Raf1 RBD region.

   Promoter

   CMV

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1P-iCLHN

   Plasmid

   #66579

   Purpose

   AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   Nanoluc-HaloTag
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-HA-Nr4a1

   Plasmid

   #160941

   Purpose

   Generation of retrovirus for the overexpression of Nr4a1

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 (Nr4a1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Promoter

   MSCV-LTR

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6-His-MBP-TEV-FnCpf1

   Plasmid

   #90094

   Purpose

   Bacterial expression plasmid for protein purification

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   FnCpf1 (humanized)
   

   Tags

   3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNon-target

   Plasmid

   #214053

   Purpose

   Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   Non-target sequence
   

   Use

   Non-target rna plasmid

   Expression

   Bacterial

   Promoter

   trc promoter

   Available Since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1P-iCAG.copGFP

   Plasmid

   #66577

   Purpose

   AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   copGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-mScarlet-Giantin

    Plasmid

    #196872

    Purpose

    Expresion of Giantin-fused to mScarlet. Used as Golgi marker

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mScarlet-Giantin (GOLGB1 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

    Plasmid

    #185446

    Purpose

    Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Inserts

    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    

    Tags

    MBP, YBBR, and ZZ

    Expression

    Insect

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDY0750 pCAG-NLS-BceINT

    Plasmid

    #179112

    Purpose

    BceINT expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCAG-NLS-BceINT
    

    Use

    CRISPR

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3 IRF7(5’UTR)-FF

    Plasmid

    #85490

    Purpose

    Firefly luciferase under the control of IRF7 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of IRF7 (Irf7 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-flag-hKDM4A-polyA

    Plasmid

    #101051

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcrDNA3.1-PRKACA

    Plasmid

    #100890

    Purpose

    Mammalian expression of PRKACA

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
    

    Expression

    Mammalian

    Mutation

    Isoform 1 wild type

    Promoter

    CMV

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TVBB C-term-mScarlet-Blast

    Plasmid

    #169221

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Blast
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55648

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7-EF-EYFP-YAP1_WT-PolyA

    Plasmid

    #112284

    Purpose

    Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    WT human YAP1-2gamma

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-hARdelta538-614

    Plasmid

    #89107

    Purpose

    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-538-614 (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR delta 538-614

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only