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Plxnb1(L)-Fc-His
Plasmid#72126PurposeExpresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb1 (Plxnb1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX302 GDF11-V5 puro
Plasmid#83097PurposeLentiviral vector for constitutive expression of human GDF11 with C-terminal V5 tagDepositorInsertGDF11 (GDF11 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-G2-ATP8-V5 (puromycin)
Plasmid#86852Purposemammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tagDepositorInsertATP8 (ATP8 Human)
UseTagsATP5G2 (G2) mitochondrial targeting signal and V5ExpressionMammalianMutationcodon optimizedPromoterCMVAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP1-S127A
Plasmid#18988DepositorInsertYes-kinase associated protein S127A mutant (YAP1 Human)
UseTagsHisExpressionMammalianMutationSerine 127 changed to Alanine, mutated protein t…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SNAP-CRY2-VHH(GFP)
Plasmid#58370PurposeFor use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MPDepositorInsertCRY2[1-498] (CRY2 Mustard Weed)
UseTagsSNAP and vhhGFP4ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 Flag JIP3b
Plasmid#53458Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
UseTagsExpressionYeastMutationPromoterT7Available sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_M337P
Plasmid#98675PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with M337PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationM337PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_A326P
Plasmid#98672PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with A326PDepositorInsertTDP-43 (TARDBP Human)
UseTags6xHisExpressionBacterialMutationA326PPromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
RH5-bio
Plasmid#47780PurposeExpresses enzymatically monobiotinylated full-length RH5 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RH5
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO(ChRger2-TS-YFP)
Plasmid#127239PurposeHigh photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CAG promoter and double floxed.DepositorInsertChRger2-TS-EYFP
UseAAVTagsEYFP and SpyTagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
VP16-hAR
Plasmid#89092Purposemammalian expression of full-length human androgen receptor with added N-terminal VP16 activation domainDepositorInserthAR (AR Human)
UseTagsVP16ExpressionMammalianMutationPromoterSV40Available sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-UBC-hAAT-IRES-ZsGreen-W
Plasmid#126702Purposelentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreenDepositorInsertAlpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
UseLentiviralTagsExpressionMutationPromoterUBCAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F4
Plasmid#55787PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)DepositorInsert3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpresses tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtAcKRS (human-opti)
Plasmid#164195PurposeTo express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansionDepositorInsertMtAcKRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationD76G; L325M; L329I; Y330F; L333A; C372FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pcDNA1 neo
Plasmid#13580DepositorInsertintegrin beta 6 (ITGB6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only
